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补料分批细胞培养

当前,有一半以上的上游细胞培养生物工艺以补料分批模式操作,在其运行过程中,细胞生长和产物形成所必需的营养物质间歇性或连续性补加。当达到细胞培养体积后,使用批量模式操作,进行收获。

生物反应器效率的不断提升,已可获得更高的细胞密度,这就需要新的澄清策略,以加强下游纯化的效率。主要的传统澄清技术,包括多步设备密集型离心、深层过滤和除菌过滤,正在向一次性使用澄清工艺转变,后者可降低过滤面积、时间以及步骤数。

To help customers optimize clarification, Repligen has developed High Productivity Harvest, an alternative application using XCell ATF® technology that can provide more efficient clarification of high-density cultures, intensify harvest and displace depth filtration while keeping core operations in Fed Batch mode.

N-1 灌流

简化并强化细胞培养而维持补料分批模式

Using award-winning alternating tangential flow (ATF) technology, XCell ATF® cell retention devices deliver high cell concentration and process intensification during cell culture. Used with the N-1 bioreactor, it achieves exceptional high cell density in the seed train step, seeding the N production bioreactor at a higher starting cell density and shortening the production bioreactor run time.

高产量收获

High Productivity Harvest uses an XCell ATF® cell retention device after peak density is achieved in a typical Fed-Batch process. Fresh media is added in a set flow rate while the harvesting of the product is initiated. This results in significant productivity and process gains.