Designed for pilot-scale purification using tangential flow filtration (TFF), the KrosFlo® KMPi System delivers precise process controls and accurate data collection.
• 100mL - 500L volume
• 0.049 m2 - 5.1 m2 filtration area
TFF for Pilot scale purification
Designed for 100mL - 500L purification applications, the KrosFlo® KMPi TFF System from Repligen is designed for robust pilot-scale downstream ultrafiltration and microfiltration.
Fully integrated and ready to use out of the box, the KMPi provides consistent and accurate flow rates with a range of 0.01 to 13.0 LPM.
KrosFlo® KMPi TFF SYSTEM
The fully integrated KrosFlo® KMPi TFF System consists of a digital peristaltic pump, multi-language interface with a graphical LCD display digital pressure monitor for automated alarm and shut-off controls, KMPi Pump head, scale for automated alarms and shut-off controls, Automatic Backpressure Valve, module stand, and proprietary KF Comm Software. The KrosFlo® KMPi provides consistent and accurate flow rates with a range of 0.01 to 13.0 LPM.
Fully integrated
Includes filters, pumps, process monitors, software and graphic display
可配置
ProConnex® custom flow paths, 4 filter module configurations, 4 membrane chemistries, 2 effective filter lengths
可放大性
Compatible with KrosFlo® TFF systems from process development to commercial manufacturing
灵活性
Use with hollow fiber filters or flat sheet cassettes
KONDUiT walkaway automation
KONDUiT add-on module for automated process control of conductivity, UV and temperature
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Automatic Back pressure control Valve
Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.
- Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the ProConnex® flow path
- Maintains a uniform TMP throughout the process run, decreasing membrane fouling
- Controls the permeate pressure and flow during microfiltration applications
- Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
- Can be set to start pinching from the open, middle, or closed position
- Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36
CONFIGURABLE Pro-Connex® FLOW PATH
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
Proprietary KF Comm Software
KF Comm Software
- Automatically collects process data
- Provides real-time overviews of the filtration process
Microsoft Surface Pro 4 with Excel
- Automatically graphs data in Excel-based software
- 21CFR Part 11 enabled when used with optional KF Comm Compliant Workbook and interface software package from OFNI Systems (ExcelSafe™)
Real-time operating parameters
- Time
- Feed Pressure
- Retentate Pressure
- Permeate Pressure
- Feed Flow Rate
- Diafiltration Flow Rate
- Permeate Flow Rate
- Feed Scale Weight
- Permeate Scale Weight
- Pump RPM
- Notes (Alarms, Process status, Functions)
- Pump Mode
Processing parameters
- Transmembrane Pressure (TMP)
- Pressure Drop (ΔP)
- Retentate Flow Rate
- Permeate Flux
- Volumetric Throughput (VT)
- Concentration Factor
- Shear
- Diafiltration Volumes
- Process Time
- UV, Conductivity, and Temperature (with KONDUiT)
Compatible Filter Modules
| Primary Application |
Volume | Inlet/ Outlet | Side Port |
Scale Length |
Effective Length (cm) | Total Length (cm) |
Surface area |
|
|---|---|---|---|---|---|---|---|---|
MiniKros Sampler |
||||||||
 |
Batch TFF of small volumes | 3 L -15 L | 0.75" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 47 70 |
490 – 1000 cm2 1000 - 2200 cm2 1600 - 3400 cm2 |
MiniKros |
||||||||
 |
Pilot-scale TFF | 5 L - 50 L | 1.5" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 46 70 |
0.16 - 0.31 m2 0.32 - 0.65 m2 0.51 - 1.0 m2 |
KrosFlo® |
||||||||
 |
Production-scale TFF | 10 – 100L | 3" TC | 1.5" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
23 46 69 |
0.78 - 1.6 m2 1.65 - 3.25 m2 2.55 - 5.10 m2 |
Compatible Flat Sheet Cassettes
KONDUiT add-on Monitor
Monitor and evaluate conductivity, UV and temperature for automated process controls. KONDUiT is an add-on module ready to use with the KrosFlo® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.
KONDUiT adds:
- End point conductivity control for UF /DF
- End point UV control for diafiltering free drug from drug delivery particles
- End point UV control for concentration of certain molecules
- UV alarm for membrane breakthrough
- A260 and A280 light sources available
*UV components sold separately
| Conductivity Range | 0.1 to 100 mS/cm |
| Temperature Range | 0-70° |
| Pressure Range | up to 75 psi (5 bar) |
| Dimensions | (L x W x H): 7¾" x 4¾" x 4½" |
| Weight | 1.6 kg |
Scalable from benchtop to production
The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KR2i TFF System
2ml - 10L
KrosFlo® KMPi TFF System
1L - 500L
KrosFlo® KTF TFF System
500L - 5000L
系统和部件
- IP 66 and NEMA 4X Enclosure
- 650 RPM pump drive with reversible motor
- 90 to 260 Vrms @ 50/60 Hz
- Standard 13 LPM Pump Head
- High Performance 17 LPM pump head (optional)
- ETL Listed and CE/UL/cUL marked
- Dimensions: 17.5 in x 11in x 13in
(445 x 230 x 330 mm) - Shipping weight: 40 lbs (18kg)
Transducers
- Measures and displays 4 pressures: Inlet Pressure, Outlet Pressure, Permeate Pressure, Transmembrane Pressure (TMP)
- User-defined pressure alarm and stop set-points
- Available with many different connector types and materials of construction
Scales
Maintain user-defined feed weight set-points, and total permeate mass during TFF processes.
Monitor
User-defined pressure alarms help ensure maximum sample recovery and membrane integrity.
KrosFlo® KR1 Auxiliary Pump
Auxiliary pumps automatically increase or decrease buffer/product addition to feed reservoir.
TangenX™ SIUS™ Flat Sheet Cassette Clamps
Stainless steel plates designed to be used with TangenX™ SIUS™ Cassettes and filter plate inserts.
