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KrosFlo® KPS TFF Systems

For use in pilot to large-scale perfusion cell culture, the KrosFlo® KPS Systems are integrated tangential flow filtration (TFF) systems.
     • 500L - 5000L volume 

TFF perfusion for commercial production

The KrosFlo® KPS Systems automatically control and maintain critical operational parameters defined by the operator during pilot and large-scale cell culture perfusion. While traditional stainless steel systems need to be cleaned in place (CIP) or steamed in place (SIP) prior to use, the KrosFlo® KPS Systems are integrated with sterile ProConnex Flow Paths for easy ready-to-operate tangential flow perfusion. 

KrosFlo® KPS TFF SYSTEMS

KrosFlo® KPS Systems are set up on a mobile cart that supports the hollow fiber filter module,  an integrated ProConnex® flow path, and an integrated TFF system that automatically controls and maintains critical operational parameters (Recirculation Shear Rate, TMP control, Permeate Flow/VVD and Reactor Volume Maintenance). Robust integrated solutions and associated connections minimize the hold up volume and provides a completely drainable process loop.

Integrated system

Hollow fiber filter, configurable ProConnex® flow path, complete TFF system mounted on a mobile cart

custom flow path

Fully integrated with sterile,custom-engineered ProConnex® flow path

可放大性

Compatible with KrosFlo® TFF perfusion systems from process development to commercial manufacturing

 

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品,以确保我们客户的业务连续性。

关于Repligen生产

Automatic Back Pressure Control Valve

Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.

  • Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the ProConnex® flow path
  • Maintains a uniform TMP throughout the process run, decreasing membrane fouling
  • Controls the permeate pressure and flow during microfiltration applications
  • Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
  • Can be set to start pinching from the open, middle, or closed position
  • Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36

CONFIGURABLE Pro-Connex® FLOW PATHS

Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration.  Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.

ProConnex® Flow Paths

Proprietary KF Comm Software

KF Comm Software

  • Automatically collects process data
  • Provides real-time overviews of the filtration process

Microsoft Surface Pro 4 with Excel

  • Automatically graphs data in Excel-based software
  • 21CFR Part 11 enabled when used with optional KF Comm Compliant Workbook and interface software package from OFNI Systems (ExcelSafe™)

Real-time operating parameters

  • Time
  • Feed Pressure
  • Retentate Pressure
  • Permeate Pressure
  • Feed Flow Rate
  • Diafiltration Flow Rate
  • Permeate Flow Rate 
  • Feed Scale Weight
  • Permeate Scale Weight
  • Pump RPM
  • Notes (Alarms, Process status, Functions)
  • Pump Mode

Processing parameters

  • Transmembrane Pressure (TMP)
  • Pressure Drop (ΔP)
  • Retentate Flow Rate
  • Permeate Flux
  • Volumetric Throughput (VT)
  • Concentration Factor
  • Shear
  • Diafiltration Volumes
  • Process Time
  • UV, Conductivity, and Temperature (with KONDUiT)

Compatible Filter Modules

  Primary
Application
Volume Inlet/ Outlet Side
Port
Scale
Length
Effective Length (cm) Total
Length (cm)
Surface
area

MiniKros Sampler

               
MiniKros-Sampler.png Batch TFF of small volumes 3 L -15 L 0.75" TC 0.75" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
25
47
70
490 – 1000 cm2
1000 - 2200 cm2
1600 - 3400 cm2

MiniKros

               
MiniKros.png Pilot-scale TFF 5 L - 50 L 1.5" TC 0.75" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
25
46
70
0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2

KrosFlo®

               
KrosFlo.png Production-scale TFF 10 – 100L 3" TC 1.5" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
23
46
69
0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2

KrosFlo® MAX

               
KrosFlo-MAX.png Production-scale TFF 100L – 1000+L 6" TC 1.5" TC R65
3 x FL
R85
49.9
67.9
107.5
73.5
91.5
131.1
4.30 - 10.40 m2
7.10 - 17.10 m2
11.30 - 11.35 m2

Scalable from benchtop to production

The KrosFlo® family of TFF Systems for perfusion cell culture supports scale up from process development to commercial manufacturing.

Components

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  • Software: Proficy HMI/SCADA-IFIX
  • Ethernet port for connection to central data acquisition system
  • 15" Color touch screen computer/display
  • Sequences available in semi-Automated or automated mode
  • Ultrasonic non-invasive Retentate Flow Meter (Permeate Flow Meter optional)
  • Nema 4X rated enclosure
  • Controller displayed reading and renderings of the flow path
  • Visual and audible alarms with safety interlocks
  • Retentate control valve that is non-invasive to the process stream
  • USB port for connection flash drive to transfer data
  • USB port can be used for connecting an external mouse or keyboard

资源

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