TFF for commercial production purification
The KrosFlo® KTF TFF Systems were developed for large-scale downstream tangential flow microfiltration or ultrafiltration of shear sensitive cells or process solutions. Both systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.
KrosFlo® KTF TFF SYSTEMS
KrosFlo® KTF Systems are set up on a mobile cart that supports the hollow fiber filter module, an integrated ProConnex® flow path, an integrated non-invasive retentate control valve, up to two peristaltic pumps, the levitating recirculating pump, a clamp on ultrasonic retentate flow meter, a flow though ultrasonic permeate flow meter and the electrical cabinet with built in 15” color touch-screen HMI. The mobile cart is made of Sanitary 304 Stainless Steel to meet NEMA 4X wash down requirements.
Integrated system
Hollow fiber filter, custom ProConnex® flow path, control valve, peristaltic and recirculating pumps, flow meters, electrical cabinet, 15” color touch-screen interface mounted on a mobile cart
custom flow path
Fully integrated with sterile,custom-engineered ProConnex® flow path
可放大性
Compatible with KrosFlo® TFF systems from process development to commercial manufacturing
灵活性
Sequences available in automated, semi-automated or manual operation modes
Easy to use
15" color touch screen computer display
Automatic Back Pressure Control Valve
Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.
- Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the ProConnex® flow path
- Maintains a uniform TMP throughout the process run, decreasing membrane fouling
- Controls the permeate pressure and flow during microfiltration applications
- Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
- Can be set to start pinching from the open, middle, or closed position
- Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36
CONFIGURABLE Pro-Connex® FLOW PATHS
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
Proprietary KF Comm Software
KF Comm Software
- Automatically collects process data
- Provides real-time overviews of the filtration process
Microsoft Surface Pro 4 with Excel
- Automatically graphs data in Excel-based software
- 21CFR Part 11 enabled when used with optional KF Comm Compliant Workbook and interface software package from OFNI Systems (ExcelSafe™)
Real-time operating parameters
- Time
- Feed Pressure
- Retentate Pressure
- Permeate Pressure
- Feed Flow Rate
- Diafiltration Flow Rate
- Permeate Flow Rate
- Feed Scale Weight
- Permeate Scale Weight
- Pump RPM
- Notes (Alarms, Process status, Functions)
- Pump Mode
Processing parameters
- Transmembrane Pressure (TMP)
- Pressure Drop (ΔP)
- Retentate Flow Rate
- Permeate Flux
- Volumetric Throughput (VT)
- Concentration Factor
- Shear
- Diafiltration Volumes
- Process Time
- UV, Conductivity, and Temperature (with KONDUiT)
Compatible Filter Modules
| Primary Application |
Volume | Inlet/ Outlet | Side Port |
Scale Length |
Effective Length (cm) | Total Length (cm) |
Surface area |
|
|---|---|---|---|---|---|---|---|---|
KrosFlo® |
||||||||
 |
Production-scale TFF | 10 – 100L | 3" TC | 1.5" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
23 46 69 |
0.78 - 1.6 m2 1.65 - 3.25 m2 2.55 - 5.10 m2 |
KrosFlo® MAX |
||||||||
 |
Production-scale TFF | 100L – 1000+L | 6" TC | 1.5" TC | R65 3 x FL R85 |
49.9 67.9 107.5 |
73.5 91.5 131.1 |
4.30 - 10.40 m2 7.10 - 17.10 m2 11.30 - 11.35 m2 |
See hollow fiber membrane types
Scalable from benchtop to production
The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KR2i TFF System
1ml - 10L
KrosFlo® KMPi TFF System
10L - 500L
KrosFlo® KTF TFF Systems
500L - 5000L
Components
KrosFlo® KTF TFF Systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.
- KTF 600 Recirculation Pump up to 50LPM @ 30 psig (2 Bar)
- KTF 650 Recirculation Pumps up to 35ml/min to 50LPM @ 30 psig (2 Bar)
- KTF 700 Recirculation Pumps up to 350ml/min to 50LPM @ 30 psig (2 Bar)
- KTF 1000 Recirculation Pump up to 110 LPM @ 43 psig (3 Bar)
- Software: Proficy HMI/SCADA-IFIX
- 304 stainless steel mobile cart with four locking swivel casters
- NEMA 4X rated enclosure, CE/UL
- Pro-Connex® Flow Path stand
- Retentate control valve that is non-invasive to the process stream
- KTF 600 Recirculation Pump up to 43 LPM at 30 psig (2 bar)
- KTF 1000 Recirculation Pump up to 110 LPM @ 43 psig (3 Bar)
- Ethernet port for connection to central data acquisition system
- 15” color touchscreen computer and display of reading and the rendering of the flow path
- Ultrasonic non-invasive retentate flow meter (or flow sensor)
- Ultrasonic flow through permeate flow meter (or flow sensor)
- Visual and audible alarms with safety interlocks
- USB port for data transfer and connection to external mouse or keyboard