KrosFlo KTF :: Repligen

KrosFlo^® KTF 切向流过滤系统工艺处理量可达2000L以上，可在商品化规模下，进行有效的切向流过滤 (TFF) 纯化。
• 10L - 2000L+ 体积

申请报价

联系方式

用于商品化生产纯化的TFF

中空纤维 TFF 工作原理

KrosFlo^® KTF TFF系统开发用于剪切敏感型细胞或工艺溶液的大规模下游切向流微滤或超滤。系统使用以PLC控制的一次性使用无脉冲磁悬浮泵。

KrosFlo^® KTF TFF 系统

KrosFlo^®KTF 系统安装在移动式推车上，其可支持中空纤维过滤器组件、整合的 ProConnex^®流路、整合的非侵入式回流控制阀、最多2台辅助蠕动泵、磁悬浮循环泵、外夹式超声波回流流量计、流通式超声波滤液流量计以及内置 15”彩色触屏HMI的电箱。移动式推车采用卫生型304不锈钢，符合NEMA4X冲洗要求。

整合式系统

中空纤维过滤器、定制化 ProConnex^® 流路、控制阀、蠕动泵和磁悬浮泵、流量计、电箱、15"彩色触屏界面整合安装于移动推车

定制流路

可无缝整合使用预灭菌的ProConnex^®工程定制流路

可放大性

兼容从工艺开发到商品化生产的KrosFlo^® TFF系统

灵活性

可选择使用内置自动化、半自动化或手动操作模式程序

简单易用

15"彩色触屏计算机显示

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品，以确保我们客户的业务连续性。

关于Repligen生产

自动背压控制阀

Repligen独特设计的KrosFlo^® 背压控制阀是进行整合式、自动化TFF工艺的关键，可降低操作员的过程管理时间以及TFF工艺时间。

当用于 ProConnex^® 流路时，可按用户确定的进样、回流、滤液压力或跨膜压（TMP），自动调节软管挤压程度
可在整个工艺运行过程中，维持均一的TMP，从而降低膜污染
可在微滤应用中控制滤液压力和流速
可随工艺过程中溶液粘度的增加或降低，相应地释放或收紧弹簧阀
可设置起始弹簧阀口距离为完全打开、中间或完全关闭状态
兼容软管尺寸13、14、16、25、HP15、17、HP24、18、HP35、36和HP36

可配置的Pro-Connex^® 流路

ProConnex^® 流路是用于切向流过滤的定制化、可配置的完整流路装置，其经工程设计过程而得以强化。该类流路装置可使用中空纤维和/或平板膜包TFF过滤器，在符合ISO指南的ISO 7级洁净室内生产，经预灭菌处理，适配于上游或下游处理应用。

ProConnex^®
流路

Single Recirculation Pump Systems

Krosflo^®KTF 200 System	Krosflo^® KTF 600 System	KrosFlo^® KTF 1000 System
One levitating recirculating pump up to 10 LPM	One levitating recirculating pump up to 45 LPM	One levitating recirculating pump up to 85 LPM
Up to 1.5 bar operation	Up to 2.0 bar operation	Up to 2.5 bar operation
Minimal recirculation volume of 500 mls with S04 filter flow path with 200 mls in recirculation loop (Total volume 700 mls)	Minimal recirculation volume of 3800 mls with K04 filter flow path with 1000 mls in recirculation loop (Total volume 4800 mls)	Minimal recirculation volume of 6500 mls with X04 filter flow path with 2100 mls in recirculation loop (Total volume 8600 mls)
Sterile TFF ProConnex^® Flow Path	Sterile TFF ProConnex^® Flow Path	Sterile TFF ProConnex^® Flow Path
MF or UF TFF operations	MF or UF TFF operations	MF or UF TFF operations
3/8” ID recirculation tubing connection	¾” ID recirculation tubing connection	1” or ¾” ID recirculation tubing connections
Non-Invassive TMP control valve	Non-Invassive TMP control valve	Non-Invassive TMP control valve
Allen Bradley PLC	Allen Bradley PLC	Allen Bradley PLC
Configurable to Delta V	Configurable to Delta V	Configurable to Delta V
iFix	iFix	iFix
iHistorian	iHistorian	iHistorian
Dream Reports	Dream Reports	Dream Reports
eSignature	eSignature	eSignature
Connectivity to Delta V	Connectivity to Delta V	Connectivity to Delta V
GMP enabled system	GMP enabled system	GMP enabled system
S and N series Spectrum^® Hollow Fiber Filters	N, K and X series Spectrum^® Hollow Fiber Filters; TangenX™ Flat Sheet Cassettes up to 5.0 m²	X series Spectrum^® Hollow Fiber Filters; TangenX™ Flat Sheet Cassettes up to 15m²
Delivery < 15 weeks	Delivery < 15 weeks	Delivery < 15 weeks

Dual Recirculation Pump SYstems

Krosflo^® KTF 650 System	Krosflo^® KTF 750 System	Krosflo^® KTF 1600 System	KrosFlo^® KTF 2000 System
Two levitating recirculating pumps	Two levitating recirculating pumps	Two levitating recirculating pumps	Two levitating recirculating pumps
One pump up to 4 LPM; One pump up to 45 LPM	One pump up to 10 LPM; One pump up to 45 LPM	One pump up to 45 LPM; One pump up to 85 LPM	Both pumps up to 85 LPM
Up to 1.5 bar operation	Up to 2.0 bar operation	Up to 2.5 bar operation	Up to 2.5 bar operation
Final recirculation volume down to 300 mls with S02 filter flow path with 100mls in recirculation loop (Total Volume 400mls)	Final recirculation volume down to 550 mls with S02- filter flow path with 100 mls in recirculation loop (Total Volume 650mls)	Final recirculation volume down to 4200 mls with N04 filter flow path with 300mls in recirculation loop (Total Volume 4500mls)	Final recirculation volume down to 50 L with two X04 filter flow path with 3 L in recirculation loop (Total Volume 56 L)
Starting Volume up to 1500L	Starting Volume up to 1500L	Starting Volume up to 2000L	Starting Volume up to 5000L
Sterile TFF Proconnex^® Flow Path (T, S, and K Modules)	Sterile TFF Proconnex^® Flow Path (S, K and X Modules)	Sterile TFF Proconnex^® Flow Path (N, K and X Modules)	Sterile TFF Proconnex^® Flow Path (X Modules)
MF or UF TFF Operations	MF or UF TFF Operations	MF or UF TFF Operations	MF or UF TFF Operations (filters run simultaneously)
TangenX™ Cassettes or Spectrum^® Hollow Fibers	TangenX™ Cassettes or Spectrum^® Hollow Fibers	TangenX™ Cassettes or Spectrum^® Hollow Fibers	TangenX™ Cassettes or Spectrum^® Hollow Fibers
1/4” and ¾” ID recirculation tubing connection	3/8” and ¾” ID recirculation tubing connection	1.0” and ¾” ID recirculation tubing connection	1” ID recirculation tubing connection
Non-Invassive TMP control valve	Non-Invassive TMP control valve	Non-Invassive TMP control valve	Non-Invassive TMP control valve
Allen Bradley PLC	Allen Bradley PLC	Allen Bradley PLC	Allen Bradley PLC
iFix	iFix	iFix	iFix
iHistorian	iHistorian	iHistorian	iHistorian
Dream Reports	Dream Reports	Dream Reports	Dream Reports
eSignature	eSignature	eSignature	eSignature
Connectivtiy to Delta V	Connectivtiy to Delta V	Connectivtiy to Delta V	Connectivtiy to Delta V
GMP enabled system	GMP enabled system	GMP enabled system	GMP enabled system
Delivery < 15 weeks	Delivery < 15 weeks	Delivery < 15 weeks	Delivery < 15 weeks

兼容过滤器组件

	主要应用	体积	进口 / 出口	侧面端口	标尺长度	有效长度 (cm)	总长度 (cm)	表面积
KrosFlo^®
	生产规模TFF	10 – 100L	3" TC	1.5" TC	1 x FL 2 x FL 3 x FL	20 41.5 65	23 46 69	0.78 - 1.6 m² 1.65 - 3.25 m² 2.55 - 5.10 m²
KrosFlo^® MAX
	生产规模TFF	100L – 1000+L	6" TC	1.5" TC	R65 3 x FL R85	49.9 67.9 107.5	73.5 91.5 131.1	4.30 - 10.40 m² 7.10 - 17.10 m² 11.30 - 11.35 m²