The KrosFlo® KTF TFF Systems were developed for large-scale downstream tangential flow microfiltration or ultrafiltration of shear sensitive cells or process solutions. Both systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.
KrosFlo® KTF Systems are set up on a mobile cart that supports the hollow fiber filter module, an integrated ProConnex® flow path, an integrated non-invasive retentate control valve, up to two peristaltic pumps, the levitating recirculating pump, a clamp on ultrasonic retentate flow meter, a flow though ultrasonic permeate flow meter and the electrical cabinet with built in 15” color touch-screen HMI. The mobile cart is made of Sanitary 304 Stainless Steel to meet NEMA 4X wash down requirements.
Hollow fiber filter, custom ProConnex® flow path, control valve, peristaltic and recirculating pumps, flow meters, electrical cabinet, 15” color touch-screen interface mounted on a mobile cart
Fully integrated with sterile,custom-engineered ProConnex® flow path
Compatible with KrosFlo® TFF systems from process development to commercial manufacturing
Sequences available in automated, semi-automated or manual operation modes
15" color touch screen computer display
Unique to Repligen, the KrosFlo® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
KF Comm Software
Microsoft Surface Pro 4 with Excel
Real-time operating parameters
|Effective Length (cm)||Total
|Production-scale TFF||10 – 100L||3" TC||1.5" TC||1 x FL
2 x FL
3 x FL
|0.78 - 1.6 m2
1.65 - 3.25 m2
2.55 - 5.10 m2
|Production-scale TFF||100L – 1000+L||6" TC||1.5" TC||R65
3 x FL
|4.30 - 10.40 m2
7.10 - 17.10 m2
11.30 - 11.35 m2
The KrosFlo® family of TFF Systems for ultrafiltration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KTF TFF Systems use a single-use non-pulsating levitating magnetic pump controlled by a PLC.