OPUS 5 - 80R :: Repligen

Leader of the Pack from bench-scale to production-scale, OPUS^® 5 - 80R Columns provide linear performance scale-up from process validation to commercial and GMP manufacturing.

Leader of the Pack

Any size, any resin, ultimate flexibility

With thousands of columns shipped globally each year, OPUS^®5 - 80RPre-packed Chromatography Columns lead the way with unparalleled flexibility in media choice, application, and size. Since launching OPUS^® Columns in 2012, Repligen is the recognized expert in packing manufacturing-scale columns today, and the innovation leader in downstream pre-packed column technology.

ANY SIZE

Volume from 0.1 L to 150 L
Bed height from 5 cm to 30 cm, configurable to the nearest milimeter

ANY RESIN

Packed with user-specified resin; over 100 resins available for OPUS^® 5 - 80R

Ultimate flexibility

Breakthrough innovative side port for resin unpacking for OPUS^® 45R, 60R, 80R

CONVENIENT

Pre-packed columns provided ready to use; no cleaning validation process; no column packing failures

成本效益

Up to 35% cost savings over self-
packed columns; up to 150 FTE hours savings per campaign; no upfront capital expense; faster campaign turnarounds

高质量生产

ISO 9001 quality manufacturing;
complete documentation package
for GMP use

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品，以确保我们客户的业务连续性。

关于Repligen生产

Scale With OPUS:
Introducing OPUS 80R

OPUS® 80R is the first and only pre-packed column enabling the purification of over 10 kilos of protein in a single cycle.

OPUS® Pre-packed Chromatography Columns offer unparalleled flexibility in media choice, application and size. Applying the most advanced pre-packed chromatography column technology available today, OPUS® columns are ready-to-use alternatives to traditional self-packed columns.

Packed with user-specified resin

Select from over 100 resins to pack into OPUS^® 5 - 80R Columns. Resins may be customer-supplied or Repligen-supplied. A convenient resin pick-up service is available upon approval.

Partial List of Resins for OPUS^® 5 - 80R

Affinity

CaptivA^®
Praesto^® AP, Jetted A50
MabSelect SuRe™ and SuRe™ LX
MabSelect™
POROS™ CaptureSelect™
AAV8, AAV9, AAVX, FcXL
AVB Sepharose^® HP
MabCapture™ A
Eshmuno^® A
Amsphere™ A3
KanCap™ A

IEX/Mixed Mode Resin

Purolite^®: SP 45, Q65, Q90
IEX Capto™ ImpRes: SP and Q
Capto™ ImpRes: adhere and MMC
IEX Capto™ : Q, S, DEAE
Capto™ adhere, Phenyl, MMC
Capto™ Core 700
IEX Sepharose^® FF: SP, Q, DEAE
IEX Sepharose^® HP: SP and Q
HIC Sepharose^® FF: Butyl and Phenyl
HIC Sepharose® HP: Butyl and Phenyl
Nuvia™ : S, cPrime, HRS

IEX POROS^®: 50HQ, 50HS, XS, XQ
HIC POROS^®: Benzyl and Ethyl
Fractogel^® TMAE, SO3-, COO, DEAE
Fractogel^® TMAE HiCap and SE HiCap
Eshmuno^®: CPX and HCX
Toyopearl^® 650M: Butyl, Phenyl,
DEAE, Super Q
Toyopearl^® 600M: Butyl, Phenyl, PPG
Toyopearl^® GigaCap 650M: S and Q
UNOsphere™: S and Q
CHT™ 40µm, 80µm Type I, Type II

All trademarks are the property of their respective owners.

Access CHT™ resin pre-packed in OPUS^® Columns

Ceramic hydroxyapatite (CHT™) resin offers unique selectivity during the purification of mAbs and recombinant proteins. OPUS^® Columns can now be pre-packed with CHT™ resin and shipped world wide with excellent performance.

Internal diameters from 5 - 45 cm
Bed heights from 5 - 30 cm

View the Full performance data

_{CHT™ is a trademark of Bio-Rad Laboratories, Inc.}

Performance maintained after shipping

Average plate count and asymmetry variance post-shipping was within 8% and 3%, respectively, of the pre-shipping value. Absolute values were within the specification window.

largest scale, broadest range

In response to the need to scale up chromatography processes, Repligen offers the largest pre-packed columns for the bioprocess industry. OPUS^® 45R, 60R and 80R Columns are the first pre-packed columns designed to meet the chromatography requirements of larger 1000L and 2000L single-use bioreactors.

Easily configured for bed height, resin, and application
Seamless and linear scale-up from smaller OPUS^® Columns
Complete documentation package for GMP use
Significant time and cost savings compared to conventional column technology

Introducing OPUS^® 80R, the latest innovation from the Leader of the Pack

The first and only 80 cm diameter pre-packed column, OPUS^® 80R can be configured with bed heights from 10-30 cm and is capable of purifying over 10 kilos of protein in a single cycle.

80 cm internal diameter
Includes recovery port for easy resin removal
Bed heights 10-30 cm, configurable to the nearest millimeter
Resin from any supplier

Scaled from the OPUS^® 45R and 60R design
Optimized flow distribution
No new product contact materials of construction
Standard 1 inch tri-clamp inlet and outlet connections
Maximum operating pressure 3 Bar, design qualified to >6 Bar

watch the webinar

	OPUS^® 45R	OPUS^® 60R	OPUS^® 80R
Internal diameter	45.7 cm	59.9 cm	79.9 cm
Column body pressure rating	3 bar	3 bar	3 bar
Bed height range	10 - 30 cm	10 - 30 cm	10 - 30 cm
Column volume: 20 cm bed height	33 L	56 L	100 L
Assembled column height	≤ 20 cm BH: 90 cm (35 in) > 20 cm BH: 116 cm (45 in)	≤ 20 cm BH: 93 cm (37 in) > 20 cm BH: 120 cm (47 in)	≤ 20 cm BH: 97 cm (38 in) > 20 cm BH: 123 cm (48 in)
Assembled column width	47 cm (18 in)	61 cm (24 in)	81 cm (32 in)
Assembled column depth	54 cm (21 in)	68 cm (27 in)	91 cm (36 in)
Inlet/Outlet flow path internal diameter	12.7 mm (0.5 in)	19.1 mm (0.7 in)	19.1 mm (0.7 in)
Inlet/Outlet port connection (per ASME BPE standards, current ed.)	½" sanitary connector	1" sanitary connector	1" sanitary connector
Estimated weight @ 20 cm BH	68 kg (150 lbs)	160 kg (350 lbs)	310 kg (690 lbs)

Innovative Resin Recovery Port

From pre-packed to unpacked

With an innovative side port that allows for easy resin unpacking, OPUS^® 45R, 60R, and 80R Columns provide the ultimate flexibility in pre-packed column technology.

Without compromising chromatographic performance or cleanability, the new feature helps mitigate the risk of implementing pre-packed columns in GMP settings, and allows for re-use of the unpacked resin in other columns.

In the event of a deviation, and if the OPUS^® pre-packed column performance cannot be recovered, the column can be unpacked and the resin can be repacked into a traditional, self-packed column.
After the intended usage of the OPUS^® pre-packed column, the column can be unpacked and resin transferred to a development or pilot setting for additional use.

观看网络研讨会

Simple unpacking procedure

A simple procedure allows for complete recovery of resin in ≤ 5 column volumes. The OPUS^® 45R, 60R, and 80R unpacking procedure includes the introduction and cycling of liquid and air between the existing column inlet and outlet ports. The process is repeated until only air is evacuated from the unpacking port.

Watch the video to see how an OPUS^® 60R Pre-packed Column is unpacked.

impact on process Economics

Substantial savings in manufacturing costs and labor are realized because OPUS^® Columns offer the benefits that come with pre-packed technology:

No up-front capital expense
Reduction of CIP procedures and cross contamination
Faster campaign turnaround time
Operational excellence (reduced labor requirements)

The OPUS^® Calculator can help you estimate how much you can save when using OPUS^® columns in your specific application.

Request a Savings Estimate

In this example, over 65% cost savings and up to 80% labor savings were achieved when using OPUS^® Columns compared to self-packed columns.

Assumptions: OPUS^® 60R Pre-packed Column versus purchase of new 60 cm diameter automated self-pack equipment (columns are dedicated to a specific molecule). Data presented are estimates and subject to change.