无可比拟的灵活性

Pre-packed Packed with any user-specified process resin

Compatible with any liquid chromatography system

内径、柱床高度和层析柱体积规格可根据特定的工艺开发需求进行定制

宽泛的可放大性

可达 10 ml 体积和 3 种直径

高性能

工艺填料批次间的可靠性能

小体积规格可降低对物料的需求,并节省宝贵的填料样品,同时获得与更大的制备型或工艺层析柱相似的性能

Click and quote OPUS® PD Pre-packed chromatography columns

 


Configure and quote pre-packed columns using the latest web-based technology.

When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes. 

Make your chromatography life easier.

Get started

 Ultimate Flexibility

Any resin, multiple column dimensions

Currently platformed in various process development projects, as well as for resin sampling by various resin vendors, over 30,000 OPUS® MiniChrom® Columns have been in used in the industry since 2007. Individual laser labeling containing Part Number and Serial Number ensure traceability to the corresponding performance data. 

  • Pre-packed with over 200 user-specified chromatography resins
  • 8 种不同层析柱直径
  • 明确定义且文件记录的装柱程序以及正确的压缩系数,可实现工艺填料的高性能
    • 折合HETP一致接近5
    • 不对称性稳定为1.1 - 1.2
    • 高达30 bar的耐压性
  • 与所有常见的液体层析系统兼容 - 1/16" 母鲁尔接头
  • Available Certificate of Analysis (CoA) for each individual column

可选构型

内径
柱床高度
柱体积
cm mm cm   mm mL
0.5 5.0 1.0 10.0 0.2
0.5 5.0 2.5 25.0 0.5
0.5 5.0 5.0 50.0 1.0
0.8 8.0 2.0 20.0 1.0
0.8 8.0 5.0 50.0 2.5
0.8 8.0 10.0 100.0 5.0
1.13 11.3 5.0 50.0 5.0
1.13 11.3 10.0 100.0 10.0

Rapid and simple AAV capture and polish DOE with new digital tools

This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan’s Fluent™ robotic workstation.

Configure your columns

播放图标 视频海报

 High Performance

工艺开发

  • 填料筛选 / 条件筛选
  • 参数优化

用于特定分子的纯化工作流的开发需要针对每一个层析步骤进行填料和操作条件评估。

使用规模缩小的层析柱配置可提高效率和通量。OPUS® MiniChrom®层析柱的性能与更大规模层析柱的性能相当,从而获得可靠且可预测的层析性能数据。使用这些小体积的规模缩小层析柱进行工艺开发,可节省宝贵的样品、成本、资源和时间。

结果可以保存在首选填料的填料库中。

Scharl et al., Trend analysis of performance parameters of pre-packed columns for protein chromatography over a time span of ten years,  Journal of Chromatography A, 1465 (2016) 63–70.

 High Performance

样品制备 / 过程分析技术 (PAT)

  • 更大的蛋白质数量

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates.  Non-purified samples require sample preparation before analysis can be executed. OPUS MiniChrom Columns enable one-step purification of multiple samples in parallel.

Use of OPUS MiniChrom Columns generates 8-400 mg of sample per run.

OPUS MiniChrom Pre-packed Columns for Process Development

Designed for bench-scale resin screening, process development,  and sample preparation, OPUS® MiniChrom® Columns are pre-packed with user-specified resin and supplied ready to use with any liquid chromatography system.  Flexible column dimension configurations (internal diameters, bed heights, volumes) allow comparative evaluation of process parameters and the construction of customized resin libraries.

Manufacturing Centers of Excellence

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品,以确保我们客户的业务连续性。

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.

地点

客户至上。

Repligen DNA 为您提供支持。我们的目标是提供卓越的客户体验,并为所有 Repligen 产品和服务高效成功的采用及实施提供支持。

  • Field Application Support
  • 客户服务
  • 现场服务工程师

还有问题吗?

规格

 

柱内径

5 mm

   

8 mm

    11.3 mm  

装填柱床高度

10 mm

25 mm

50 mm

20 mm

50 mm

100 mm

50 mm

100 mm

柱体积

0.2 mL

0.5 mL

1.0 mL

1.0 mL

2.5 mL

5.0 mL

5.0 mL

10.0 mL

内截面积

0.2 cm2

   

0.5 cm2

   

1.0 cm2

 

接头

手紧接头 1/16",母鲁尔 (10-32)

柱材料

聚丙烯

化学稳定性

所有常用水性缓冲液,pH 1 – 14,有机溶剂**。
避免使用卤代有机溶剂、己烷

柱储存溶液

AIEC 和 CIEC 层析柱: 20 %乙醇 + 150 mM 氯化钠
HIC、SEC、亲和和MMC层析柱: 20 % 乙醇
CHT、CFT 层析柱 (Bio-Rad):20 % 乙醇 + 10 mM 磷酸钠,pH 6.8
ProSep® 层析柱(Millipore):1 % 苄醇+ 0.1M 醋酸钠,pH 5.1

推荐 储存温度

AIEC、CIEC、HIC、SEC 和 MMC:+4°C - +30°C
亲和层析柱:+2°C - +8°C

工作温度

+4°C - +30°C

最高工作 压力

最高30bar

流速

16 – 1000 cm/h**

标签

激光印刻在每根层析柱柱体上。标签包含:

  • 产品编号
  • 序列号
  • 填料名称
  • 层析柱尺寸 (ID x H、V)
  • 液流方向

* 还需要考虑所预装的层析填料的化学稳定性。更多信息,请参考厂商的填料规格说明。