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Screening and Sample Preparation
OPUS® MiniChrom® Pre-packed Columns

 

OPUS® MiniChrom® Columns are designed for bench-scale resin screening, process development and sample preparation.

申请报价

Shop opus® Minichrom® Columns for AAV

Pre-packed Columns for process development

Designed for bench-scale resin screening, process development,  and sample preparation, OPUS® MiniChrom® Columns are pre-packed with user-specified resin and supplied ready to use with any liquid chromatography system.  Flexible column dimension configurations (internal diameters, bed heights, volumes) allow comparative evaluation of process parameters and the construction of customized resin libraries.

OPUS® MiniChrom® Pre-packed Columns

Currently platformed in various process development projects, as well as for resin sampling by various resin vendors, over 30,000 OPUS® MiniChrom® Columns have been in used in the industry since 2007.  Individual laser labeling containing Part Number and Serial Number ensure traceability to the corresponding performance data. 

  • 可预装用户指定的层析填料 - 已超200种填料类型
  • 8 different column dimensions
  • Defined, documented packing procedures with correct compression factor to deliver high performance from process resins ​​​​
    • Reduced HETP consistent near 5
    • Asymmetry stable at 1.1 to 1.2
    • Up to 30 bar pressure resistance
  • Compatible with all common liquid chromatography systems - 1/16" female connectors
  • A Certificate of Analysis (CoA) for each individual column is available on request

查看填料列表

可选构型

     

内径

 柱床高度 柱体积
cm mm cm   mm mL  
0.5 5.0 1.0 10.0 0.2  
0.5 5.0 2.5 25.0 0.5  
0.5 5.0 5.0 50.0 1.0  
0.8 8.0 2.0 20.0 1.0  
0.8 8.0 5.0 50.0 2.5  
0.8 8.0 10.0 100.0 5.0  
1.13 11.3 5.0 50.0 5.0  
1.13 11.3 10.0 100.0 10.0  
OPUS MiniChrom Columns

Internal diameter x Bed Height (cm)

便利性

预装层析柱，即取即用

灵活性

Packed with any user-specified process resin; Compatible with any liquid chromatography system

Variable configurations

Internal diameter, bed height, and column volume configurations enable customization to specific process development needs

High reproducibility

Reliable performance from process resins batch to batch

Broad scalability

Up to 10 ml volume and 3 diameters

成本效益

Small configuration drives reduced material needs, and saves valuable resin samples while delivering similar performance to larger preparative or process columns

 

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品，以确保我们客户的业务连续性。

关于Repligen生产

proven Applications

​Process Development

  • Resin screening / Condition screening
  • Parameter optimization

The development of a purification workflow for a specific molecule requires evaluation of the resin and the operating conditions for each chromatography step.

Using a scale-down chromatography column configuration drives efficiency and higher throughput. The performance of OPUS® MiniChrom® Columns is equivalent to the performance of larger columns, yielding reliable and predictive chromatography performance data. Using these smaller scale-down columns for process development saves valuable samples, cost, resources and time.

Results can be saved in a resin library of preferred resins.

Scharl et al., Trend analysis of performance parameters of pre-packed columns for protein chromatography over a time span of ten years,  Journal of Chromatography A, 1465 (2016) 63–70.

Sample preparation / Process Analytical Technology (PAT)

  • Larger protein quantities

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates.  Non-purified samples require sample preparation before analysis can be executed. OPUS® MiniChrom® Columns enable one-step purification of multiple samples in parallel.

Use of OPUS® MiniChrom® Columns generates 8-400 mg of sample per run.

OPUS MiniChrom Columns

Specifications

柱内径

5 mm

    

8 mm

     11.3 mm  

装填柱床高度

10 mm

 25 mm

50 mm

20 mm

50 mm

100 mm

50 mm

 100 mm

Column volume

0.2 mL

0.5 mL

1.0 mL

1.0 mL

2.5 mL

5.0 mL

5.0 mL

 10.0 mL

内截面积

0.2 cm2

    

0.5 cm2

    

1.0 cm2

  

接头

Fingertight 1/16", female (10-32)

Column material

聚丙烯

化学稳定性

All commonly used aqueous buffers, pH 1 – 14, organic solvents*.
Avoid the use of halogenated organic solvents, hexane

柱储存溶液

AIEC 和 CIEC 层析柱： 20 %乙醇 + 150 mM 氯化钠
HIC、SEC、亲和和MMC层析柱： 20 % 乙醇
CHT、CFT 层析柱 (Bio-Rad)：20 % 乙醇 + 10 mM 磷酸钠，pH 6.8
ProSep® 层析柱(Millipore):1 % 苄醇+ 0.1M 醋酸钠，pH 5.1

推荐 储存温度

AIEC、CIEC、HIC、SEC 和 MMC：+4°C - +30°C
亲和层析柱：+2°C - +8°C

工作温度

+4°C - +30°C

最高工作 压力

最高30bar

Flow velocity

16 – 1000 cm/h**

Labelling

Laser-etched on every individual column body. Label contains:

  • Product number
  • Serial number
  • Resin name
  • Column dimensions (ID x H, V)
  • Flow direction

* The chemical stability of the packed chromatography resin also has also to be taken into consideration. For more information, please refer to the manufacturer’s resin specification.

资源

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