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Screening and Sample Preparation
OPUS® RoboColumn® Pre-packed Columns

 

OPUS® RoboColumn® miniaturized pre-packed columns are on a 96-well plate, designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.

Automated parallel Chromatography

OPUS RoboColumn

Shorten process development time significantly by performing parallel operation of OPUS® RoboColumn® pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design).

OPUS® RoboColumn® Pre-packed Columns

Used in the industry since 2007, OPUS® RoboColumn® pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.

Up to 96 RoboColumn® pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements.  Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.

  • 可预装用户指定的层析填料 - 已超300种填料类型
  • Consistently packed with correct axial compression
  • Operating linear flow-rate controlled by dispensing speed of the liquid handling workstation
  • Demonstrated scale-up and scale-down due to constant residence time 
  • Large number of samples per time, ability to multiplex

查看填料列表

可选构型

     
内径 柱床高度 Column
Volume
cm mm cm mm mL µL
0.5 5.0 0.25 2.5 0.05 50
0.5 5.0 0.50 5.0 0.10 100
0.5 5.0 1.00 10.0 0.20 200
0.5 5.0 2.25 22.5 0.45 450
0.5 5.0 2.50 25.0 0.50 500
0.5 5.0 3.00 30.0 0.60 600


Minimum order is one row of 8 OPUS®  RoboColumn® pre-packed columns  with the same resin and column dimension.

OPUS RoboColumn

OPUS® RoboColumn®
internal design

OPUS RoboColumn

OPUS® RoboColumn®
96-well plate

OPUS® RoboColumn®
5 mm ID x 30 mm H; V = 600 µL

OPUS® RoboColumn®
5 mm ID x 10 mm H; V = 200 µL

便利性

预装层析柱,即取即用

灵活性

Packed with user-specified chromatography resin, multiple column volumes

Automated

Use with Tecan and Perkin Elmer robotic fluid handlers

Fast

Process up to 8 OPUS® RoboColumn® pre-packed columns in parallel, shorten development time

成本效益

Reduces resource requirements

 

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品,以确保我们客户的业务连续性。

关于Repligen生产

Designed for robotic Fluid Handlers

Compatible with both Tecan and PerkinElmer platforms, the preferred supplier for OPUS®RoboColumn® automation is the Tecan Freedom EVO® robotic workstation.

  • Simultaneous automated step-gradient elution into a 96-well plate
  • Subsequent analysis in an integrated plate reader
  • Flexible software wizard 

The combination of OPUS® RoboColumn® and the Tecan Freedom EVO® robotic workstation delivers a complete integrated platform for state-of-the-art small-scale protein purification.

OPUS RoboColumn

screening and sample preparation Applications

工艺开发

  • Resin screening / Condition screening

Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step. Using OPUS® RoboColumn® on a Tecan Freedom EVO® generated up to 96 pseudo-chromatograms (1536 data-points) in 1.5 days when comparing 12 CIEX resins at 8 different pH 3.7-7.0.

  • 8 chromatographic separations in parallel with 16 fractions each in 16 minutes
  • 96 individual buffers in 10 minutes

Sample Preparation / Process Analytical Technology (PAT)

  • In-process fermentation monitoring
  • Protein characterization
  • Drug discovery

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates.  Non-purified samples require sample preparation before analysis can be executed. OPUS® RoboColumn® enables one-step purification of multiple samples in parallel.

Using the OPUS® RoboColumn® on a Tecan Freedom EVO® generates 2mg to 24mg API (Active Pharmaceutical Ingredient) per RoboColumn® run.

Specifications

Plate size

128.3 L x 86.0 W x 14.0 mm H

Plate material

Polyoxymethylene copolymer

Number of wells

96

Column volume

50 µL

100 µL

200 µL

450 µL

500 µL

600 µL

Bed height

2.5 mm

5.0 mm

10.0 mm

22.5 mm

25.0 mm

30.0 mm

Column inner diameter

5 mm

Column material

聚丙烯

化学稳定性

All commonly used aqueous buffers, pH 1 – 14, organic solvents*.  Avoid the use of halogenated organic solvents, hexane

柱储存溶液

AIEC 和 CIEC 层析柱: 20 %乙醇 + 150 mM 氯化钠
HIC、SEC、亲和和MMC层析柱: 20 % 乙醇
CHT、CFT 层析柱 (Bio-Rad):20 % 乙醇 + 10 mM 磷酸钠,pH 6.8
ProSep® 层析柱(Millipore):1 % 苄醇+ 0.1M 醋酸钠,pH 5.1

推荐 储存温度

AIEC、CIEC、HIC、SEC 和 MMC:+4°C - +30°C
亲和层析柱:+2°C - +8°C

工作温度

+4°C - +30°C

最高工作 压力

Up to 8.0 bar

Flow velocity

16 – 1000 cm/h**

Labelling

Laser-etched on every individual column body. Label contains:

  • Product number
  • Resin name
  • Column volume in µL

* Note: The chemical stability of the packed chromatography resin has also to be taken into consideration. For more information please refer to the manufacturer’s resin specification.
** The Liquid Transfer menu of the Te‑Chrom™ Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te‑Chrom™ Wizard Software Manual, 396076, en, V1.0).