OPUS RoboColumn :: Repligen

OPUS^®RoboColumn^® miniaturized pre-packed columns are on a 96-well plate, designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.

Automated parallel Chromatography

Shorten process development time significantly by performing parallel operation of OPUS^® RoboColumn^® pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design).

OPUS^® RoboColumn^®Pre-packed Columns

Used in the industry since 2007, OPUS^® RoboColumn^® pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.

Up to 96 RoboColumn^® pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.

可预装用户指定的层析填料 - 已超300种填料类型
Consistently packed with correct axial compression
Operating linear flow-rate controlled by dispensing speed of the liquid handling workstation
Demonstrated scale-up and scale-down due to constant residence time
Large number of samples per time, ability to multiplex

查看填料列表

可选构型


内径		柱床高度		Column Volume
cm	mm	cm	mm	mL	µL
0.5	5.0	0.25	2.5	0.05	50
0.5	5.0	0.50	5.0	0.10	100
0.5	5.0	1.00	10.0	0.20	200
0.5	5.0	2.25	22.5	0.45	450
0.5	5.0	2.50	25.0	0.50	500
0.5	5.0	3.00	30.0	0.60	600

Minimum order is one row of 8 OPUS^® RoboColumn^®pre-packed columns with the same resin and column dimension.

OPUS^® RoboColumn^®
internal design

OPUS^® RoboColumn^®
96-well plate

OPUS^® RoboColumn^®
5 mm ID x 30 mm H; V = 600 µL

OPUS^® RoboColumn^®
5 mm ID x 10 mm H; V = 200 µL

便利性

预装层析柱，即取即用

灵活性

Packed with user-specified chromatography resin, multiple column volumes

Automated

Use with Tecan and Perkin Elmer robotic fluid handlers

Fast

Process up to 8 OPUS^® RoboColumn^® pre-packed columns in parallel, shorten development time

成本效益

Reduces resource requirements

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品，以确保我们客户的业务连续性。

关于Repligen生产

Designed for robotic Fluid Handlers

Compatible with both Tecan and PerkinElmer platforms, the preferred supplier for OPUS^®RoboColumn^® automation is the Tecan Freedom EVO^® robotic workstation.

Simultaneous automated step-gradient elution into a 96-well plate
Subsequent analysis in an integrated plate reader
Flexible software wizard

The combination of OPUS^® RoboColumn^®and the Tecan Freedom EVO^® robotic workstation delivers a complete integrated platform for state-of-the-art small-scale protein purification.

screening and sample preparation Applications

工艺开发

Resin screening / Condition screening

Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step. Using OPUS^® RoboColumn^® on a Tecan Freedom EVO^® generated up to 96 pseudo-chromatograms (1536 data-points) in 1.5 days when comparing 12 CIEX resins at 8 different pH 3.7-7.0.

8 chromatographic separations in parallel with 16 fractions each in 16 minutes
96 individual buffers in 10 minutes

Sample Preparation / Process Analytical Technology (PAT)

In-process fermentation monitoring
Protein characterization
Drug discovery

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples require sample preparation before analysis can be executed. OPUS^® RoboColumn^®enables one-step purification of multiple samples in parallel.

Using the OPUS^® RoboColumn^® on a Tecan Freedom EVO^® generates 2mg to 24mg API (Active Pharmaceutical Ingredient) per RoboColumn^®run.

Specifications

Plate size	128.3 L x 86.0 W x 14.0 mm H
Plate material	Polyoxymethylene copolymer
Number of wells	96
Column volume	50 µL	100 µL	200 µL	450 µL	500 µL	600 µL
Bed height	2.5 mm	5.0 mm	10.0 mm	22.5 mm	25.0 mm	30.0 mm
Column inner diameter	5 mm
Column material	聚丙烯
化学稳定性	All commonly used aqueous buffers, pH 1 – 14, organic solvents*. Avoid the use of halogenated organic solvents, hexane
柱储存溶液	AIEC 和 CIEC 层析柱： 20 %乙醇 + 150 mM 氯化钠 HIC、SEC、亲和和MMC层析柱： 20 % 乙醇 CHT、CFT 层析柱 (Bio-Rad)：20 % 乙醇 + 10 mM 磷酸钠，pH 6.8 ProSep^® 层析柱(Millipore):1 % 苄醇+ 0.1M 醋酸钠，pH 5.1
推荐储存温度	AIEC、CIEC、HIC、SEC 和 MMC：+4°C - +30°C 亲和层析柱：+2°C - +8°C
工作温度	+4°C - +30°C
最高工作压力	Up to 8.0 bar
Flow velocity	16 – 1000 cm/h**
Labelling	Laser-etched on every individual column body. Label contains: Product number Resin name Column volume in µL

* Note: The chemical stability of the packed chromatography resin has also to be taken into consideration. For more information please refer to the manufacturer’s resin specification.

** The Liquid Transfer menu of the Te‑Chrom™ Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te‑Chrom™ Wizard Software Manual, 396076, en, V1.0).

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OPUS® RoboColumn® miniaturized pre-packed columns are on a 96-well plate, designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.