Novel SARS CoV-2 specific affinity resin enables a high purity capture step, decreased process time, and yield improvement for the commercial production of COVID-19 vaccines.
for the purification of COVID-19 vaccines
Novel affinity resin for the purification of the SARS CoV-2 Spike Protein for vaccine development and manufacturing
The result of close work with Navigo Proteins on the ligand development and partnership with Purolite Life Sciences on Praesto® agarose bead technology, the new NGL COVID-19 Spike Protein Affinity Resin from Repligen binds the SARS CoV-2 Spike Protein receptor binding domain (RBD) to yield exceptional purity in a single chromatography step. The product meets all the expected requirements of a high-performance resin, including very high selectivity, high dynamic binding capacities and caustic stability over multi-cycle use.
The resin is available in off-the shelf, pre-packed and pre-qualified OPUS® Columns for rapid implementation as well as in loose resin formats.
Single-step affinity chromatography with high resolution
NGL COVID-19 Spike Protein Affinity Resin key features include:
- 含有SARS-CoV-2刺突蛋白RBD的产品的单步纯化*
- Compatible with 0.1N NaOH for CIP
- High binding capacity **
* Targets SARs CoV-2 spike protein variants containing the RBD. Examples include: S protein (S1/S2), S1 Protein, RBD, S Protein Trimer. Development data suggest that purification of whole inactiviated virus, virus like particles (VLPs), and adenovirus may not yield satisfactory results.
**Actual DBC dependent on S protein variant & size
optimized bead chemistry delivers exceptional purity
NGL COVID-19 Spike Protein Affinity Resin delivers >90% purity in one chromatography step.
以HEK293细胞表达的SARS-CoV-2刺突蛋白受体结合结构域（RBD）以NGL COVID-19刺突蛋白亲和填料纯化的SDS-PAGE凝胶图。
Available Pre-packed in OPUS® Columns for quick deployment
NGL COVID-19刺突蛋白亲和填料可以经质量确认的现货OPUS® 预装柱形式提供，以简化并加快GMP使用，同时，也提供“散装”填料形式。
使用预装、预质量确认的OPUS®层析柱，可使您在数周内从开发进入生产规模。
