Featuring a low shear magnetic levitating pump for improved cell viability, the KrosFlo® KML TFF System is ideal for upstream perfusion managing 1.5L to 100L working volume bioreactors. Combined with gamma sterilized single-use ProConnex® Flow Paths, the KrosFlo® KML System delivers high protein production during lengthy TFF perfusion operations.
The KrosFlo® KML System enables faster process development facilitating multiple tests and processes, accurate process definition and execution consistency. Fully integrated and ready to use out of the box, the system includes precise and reproducible control and documentation of TFF processes.
The KrosFlo® KML TFF System is a completely integrated system with a color touchscreen to display key parameters. There are visual and audible alarms with safety interlocks to ensure process parameters are maintained. The system comes with two auxiliary peristaltic pumps, two auxiliary scales for precise the management of the flow dynamics are sold separately. A retentate control valve manages TMP by adjusting the pinch distance between the plunger and the tubing, and an ultrasonic retentate flow meter that clamps on the tubing. To prevent cells from settling and forming a gel layer on the membrane lumen, the KrosFlo® KML System adjusts through a PID control loop from the flow meter. Further advancements include automatic back pulsing features and USB port for real time data collection.
Includes filters, pumps, scales, process monitors, flow meters, software and graphic display
TFF Perfusion, 5 additional TFF modes, 1 manual mode. Scale sizes selected by the user
2 filter module configurations, 4 membrane chemistries, 3 effective filter lengths
Pressures, flows, weights and process overview with end user set-point functionality.
兼容从工艺开发到商品化生产的 KrosFlo® TFF 灌流系统
Perfusion mode, five system process control modes and one manual operation mode are available with the KrosFlo® KML TFF System.
|Perfusion Mode||Cell Culture perfusion|
|手动模式||Simple concentration of the process solution|
Simple concentration of the process solution
|C/D||浓缩 / 洗滤||工艺溶液先后浓缩和洗滤|
|C/D/C||浓缩 / 洗滤 / 浓缩||工艺溶液初步浓缩后，以一种缓冲液洗滤，然后进行二次浓缩|
|C/D/D/C||浓缩 / 洗滤 / 洗滤 / 浓缩||工艺溶液初步浓缩后，以两种不同的缓冲液先后洗滤，然后进行二次浓缩|
|体积||进口 / 出口||侧面
|小体积批量TFF||3 L -15 L||0.75" TC||0.75" TC||1 x FL
2 x FL
3 x FL
|490 – 1000 cm2
1000 - 2200 cm2
1600 - 3400 cm2
|中试规模TFF||5 L - 50 L||1.5" TC||0.75" TC||1 x FL
2 x FL
3 x FL
|0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2
The KrosFlo® family of TFF Systems for perfusion cell culture supports scale up from process development to commercial manufacturing.
Pro-Connex® Flow Path Stand
Designed to hold gamma sterilized single-use Pro-Connex® Flow Path assemblies
2 Integrated Peristaltic Pumps
Retentate Control Valve
Ultrasonic Single Pass Retentate Flow Meter