Featuring a low shear magnetic levitating pump for improved cell viability, the KrosFlo® KML TFF System is ideal for upstream perfusion managing 1.5L to 100L working volume bioreactors. Combined with gamma sterilized single-use ProConnex® Flow Paths, the KrosFlo® KML System delivers high protein production during lengthy TFF perfusion operations.
The KrosFlo® KML System enables faster process development facilitating multiple tests and processes, accurate process definition and execution consistency. Fully integrated and ready to use out of the box, the system includes precise and reproducible control and documentation of TFF processes.
The KrosFlo® KML TFF System is a completely integrated system with a color touchscreen to display key parameters. There are visual and audible alarms with safety interlocks to ensure process parameters are maintained. The system comes with two auxiliary peristaltic pumps, two auxiliary scales for precise the management of the flow dynamics are sold separately. A retentate control valve manages TMP by adjusting the pinch distance between the plunger and the tubing, and an ultrasonic retentate flow meter that clamps on the tubing. To prevent cells from settling and forming a gel layer on the membrane lumen, the KrosFlo® KML System adjusts through a PID control loop from the flow meter. Further advancements include automatic back pulsing features and USB port for real time data collection.
Includes filters, pumps, scales, process monitors, flow meters, software and graphic display
TFF Perfusion, 5 additional TFF modes, 1 manual mode. Scale sizes selected by the user
2 filter module configurations, 4 membrane chemistries, 3 effective filter lengths
Pressures, flows, weights and process overview with end user set-point functionality.
Out of the box functionality, short time to system proficiency
Compatible with KrosFlo® TFF perfusion systems from process development to commercial manufacturing
Enhanced through an engineering design process, ProConnex® Flow Paths are customized, configurable and complete assemblies for tangential flow filtration. Designed for use with hollow fiber and/or flat sheet TFF systems, the assemblies are sterile, fit for upstream or downstream processing and manufactured in compliance with ISO guidelines in an ISO 7 clean room.
Perfusion mode, five system process control modes and one manual operation mode are available with the KrosFlo® KML TFF System.
|Perfusion Mode||Cell Culture perfusion|
|Manual Mode||Simple concentration of the process solution|
Simple concentration of the process solution
|C/D||Concentration / Diafiltration||Concentration and then diafiltration of the process solution in succession|
|C/D/C||Concentration / Diafiltration / Concentration||Diafiltration of the process solution with one buffer before concentration of the process solution a second time|
|C/D/D/C||Concentration / Diafiltration / Diafiltration / Concentration||Diafiltration of the process solution with two different buffers in succession before concentration of the process solution a second time|
|CFC||Constant Feed Concentration||Concentration of the solution while maintaining a constant feed weight|
|Effective Length (cm)||Total
|Batch TFF of small volumes||3 L -15 L||0.75" TC||0.75" TC||1 x FL
2 x FL
3 x FL
|490 – 1000 cm2
1000 - 2200 cm2
1600 - 3400 cm2
|Pilot-scale TFF||5 L - 50 L||1.5" TC||0.75" TC||1 x FL
2 x FL
3 x FL
|0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2
The KrosFlo® family of TFF Systems for perfusion cell culture supports scale up from process development to commercial manufacturing.
Pro-Connex® Flow Path Stand
Designed to hold gamma sterilized single-use Pro-Connex® Flow Path assemblies
2 Integrated Peristaltic Pumps
Retentate Control Valve
Ultrasonic Single Pass Retentate Flow Meter