您使用的浏览器不受支持。
本网站的某些功能可能无法正常运行。为获得最佳的用户体验,请使用Chrome、Firefox、Safari或Edge浏览本网站。

KrosFlo® KML TFF System

The KrosFlo® KML System is designed for upstream bioreactor perfusion using tangential flow filtration (TFF), with a single-use, non-pulsating, levitating magnetic pump.
     • 1.5L - 100L volume

Featuring a low shear magnetic levitating pump for improved cell viability, the KrosFlo® KML TFF System is ideal for upstream perfusion managing 1.5L to 100L working volume bioreactors.  Combined with gamma sterilized single-use ProConnex® Flow Paths, the KrosFlo® KML System delivers high protein production during lengthy TFF perfusion operations.

The KrosFlo® KML System enables faster process development facilitating multiple tests and processes, accurate process definition and execution consistency. Fully integrated and ready to use out of the box, the system includes precise and reproducible control and documentation of TFF processes.

KrosFlo® KML TFF SYSTEM

The KrosFlo® KML TFF System is a completely integrated system with a color touchscreen to display key parameters. There are visual and audible alarms with safety interlocks to ensure process parameters are maintained. The system comes with two auxiliary peristaltic pumps, two auxiliary scales for precise the management of the flow dynamics are sold separately. A retentate control valve manages TMP by adjusting the pinch distance between the plunger and the tubing, and an ultrasonic retentate flow meter that clamps on the tubing. To prevent cells from settling and forming a gel layer on the membrane lumen, the KrosFlo® KML System adjusts through a PID control loop from the flow meter. Further advancements include automatic back pulsing features and USB port for real time data collection.

完全整合

Includes filters, pumps, scales, process monitors, flow meters, software and graphic display

 

Multiple modes

TFF Perfusion, 5 additional TFF modes, 1 manual mode. Scale sizes selected by the user

可配置

2 filter module configurations, 4 membrane chemistries, 3 effective filter lengths

easy User controls

Pressures, flows, weights and process overview with end user set-point functionality. 

简单易用

开箱即用,短时间即可熟练操作

可放大性

兼容从工艺开发到商品化生产的 KrosFlo® TFF 灌流系统

 

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品,以确保我们客户的业务连续性。

关于Repligen生产

CONFIGURABLE ProConnex® FLOW PATH

ProConnex® 流路是用于切向流过滤的定制化、可配置的完整流路装置,其经工程设计过程而得以强化。该类流路装置可使用中空纤维和/或平板膜包TFF过滤器,在符合ISO指南的ISO 7级洁净室内生产,经预灭菌处理,适配于上游或下游处理应用。

ProConnex®
流路

Automated Process Control Modes

Perfusion mode, five system process control modes and one manual operation mode are available with the KrosFlo® KML TFF System.

Mode 主要应用  
  Perfusion Mode Cell Culture perfusion  
  手动模式 Simple concentration of the process solution  
C 浓缩模式 

Simple concentration of the process solution
Automated flushing of the hollow fiber filter

 
C/D 浓缩 / 洗滤 工艺溶液先后浓缩和洗滤  
C/D/C 浓缩 / 洗滤 / 浓缩 工艺溶液初步浓缩后,以一种缓冲液洗滤,然后进行二次浓缩  
C/D/D/C 浓缩 / 洗滤 / 洗滤 / 浓缩 工艺溶液初步浓缩后,以两种不同的缓冲液先后洗滤,然后进行二次浓缩  
CFC 恒定进样浓缩 在维持恒定进样重量条件下,进行溶液浓缩  

兼容过滤器组件

  主要
应用
体积 进口 / 出口 侧面
端口
标尺
长度
有效长度 (cm)
长度 (cm)

面积

MiniKros Sampler

               
MiniKros-Sampler.png 小体积批量TFF 3 L -15 L 0.75" TC 0.75" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
25
47
70
490 – 1000 cm2
1000 - 2200 cm2
1600 - 3400 cm2

MiniKros

               
MiniKros.png 中试规模TFF 5 L - 50 L 1.5" TC 0.75" TC 1 x FL
2 x FL
3 x FL
20
41.5
65
25
46
70
0.16 - 0.31 m2
0.32 - 0.65 m2
0.51 - 1.0 m2

See Hollow Fiber Membrane Types

可从实验室规模放大至生产

The KrosFlo® family of TFF Systems for perfusion cell culture supports scale up from process development to commercial manufacturing.

KrosFlo® KML System
1.5L - 100L

KrosFlo® KPS 系统
500 L -5000 L

部件

全部展开
  • Flow Range 350 ml/min to 13 LPM at 7000 RPM @ 15 psig (1 bar)
  • 0-9000 RPM range with 300 watt power supply
  • FDA approved materials, USP Class VI, Animal BSE/TSE Free Material
  • PLC with color 8.4" touchscreen HMI
  • 0-40° C Operating Range
  • CE/UL/cUL marked
  • Automation Direct DL06 PLC with 800x600 Resolution HMI
  • Splash-proof

Integrated Detachable
Pro-Connex® Flow Path Stand

Designed to hold gamma sterilized single-use Pro-Connex® Flow Path assemblies

2 Integrated Peristaltic Pumps

  • Flow ranges from 0.16 to 347 ml/min
  • Permeate pump sequenced for back-pulsing in the Perfusion mode
  • RS232 communication
  • Integrated power within single connection to KML

Retentate Control Valve

  • Non-invasive to process stream
  • 4-20 mA control

Ultrasonic Single Pass Retentate Flow Meter

  • Measures flow up to 20LPM +/- 5%
  • Calibrated for Dow Pharma tubing at 35°C

资源

全部展开