More Potent than Insulin
LONG® R3 IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) specifically engineered to enhance cell culture performance.
- Ideal for systems utilizing serum-free or low-level serum applications
- Increased cell density, higher viability and extended culture duration
Better science for better cell culture
All mammalian cells secrete IGF Binding Proteins (IGFBPs) which bind to and inhibit native IGF-I. The substitution of an arginine for glutamic acid at position three in LONG® R3 IGF-I, in conjunction with the 13 amino acid N-terminal extension peptide, results in > 1000 fold reduced affinity for IGFBPs, enhancing its bio-availability and effectiveness in comparison to native IGF-I.
Unlike insulin, LONG® R3 IGF-I specifically targets and activates the type I IGF Receptor (IGF-IR) resulting in greater activation of downstream signaling cascades responsible for proliferation and inhibiting apoptosis. Greater activation of these key pathways results in increased cell density, higher viability and extended cell culture productivity.
To learn more about the use of LONG® R3 IGF-I in stem cell and cell therapy applications, please contact Repligen.
Cell culture supplements are essential for long-term growth and productivity of cell lines in serum-free media formulations. Both insulin and LONG® R3 IGF-I, a more potent supplement that directly targets and activates the IGF-I receptor, are employed by the biopharmaceutical industry today to support the growth of recombinant cell lines. This webinar will show how these two supplements, used separately and in combination, impact viable cell density and IgG production of multiple CHO cell lines. It will demonstrate how a combination of LONG® R3 IGF-I and insulin results in less protein supplementation and, therefore, less potential impact on downstream processing.
Improved volumetric productivity
LONG® R3 IGF-I has been shown to improve volumetric productivity in CHO cell culture by up to 62% over cell culture with no supplementation and 40% over cell culture supplemented with insulin.
LONG® R3 IGF-I Cell Culture Supplement
LONG® R3 IGF-I is a critically important mitogenic growth factor often used in mammalian cell culture to increase yield significantly. Such growth factors are fundamental supplements to chemically-defined media.
LONG® R3 IGF-I is compatible with multiple cell types. Any mammalian cell type that responds to insulin in cell culture has the potential to respond to LONG® R3 IGF-I.
- CHO 克隆
- HEK 293
LONG® R3 IGF-I is available in two formats, both stable at 2-8°C.
LONG® R3 IGF-I can also be milled into cell culture media for additional flexibility and convenience.
Since LONG® R3 IGF-I ships at ambient conditions and stored at 2-8°C, cold chain shipping and storage costs can be significantly reduced.
- 5 mg (样品规格*)
- 50 mg
- 5 mL (样品规格*)
- 100 mL
- 250 mL
- 500 mL
- 1L (1000 mL)
LONG® R3 IGF-I ELISA Kit
The only commercially available kit for the quantitation of LONG® R3 IGF-I, the kit is fast, accurate, and easy to use with both cell culture and drug substance samples. The sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
- LONG® R3 IGF-I coated strip plate, 96 wells
- LONG® R3 IGF-I Standard
- LoBind® 1.5mL EP管