LONG® R³ IGF-I 细胞培养添加物
LONG® R3 IGF-I (LONG R3) 是人胰岛素样生长因子 I (IGF-I) 的重组类似物，专为提高细胞培养性能而设计，在细胞培养中的效力是胰岛素的 200 倍，稳定性是胰岛素的 2 倍。
干粉或液体形式，可用于多种细胞类型
Animal-free manufacturing process
Comprehensive supply chain risk management
HIGH PERFORMANCE
More Potent than Insulin
LONG® R3 IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) specifically engineered to enhance cell culture performance.
- 相比胰岛素或天然IGF-I，更高的体外生物学效价
- Ideal for systems utilizing serum-free or low-level serum applications
- Increased cell density, higher viability and extended culture duration
Request a sample today and boost your cell productivity
Try a sample of LONG R3-IGF-I in your cell culture process to see how it can enhance cell performance and productivity. Start improving your cell performance today.
HIGH PERFORMANCE
Better science for better cell culture
LONG® 技术包括生长因子与生长激素序列部分的融合。该融合可提高生产因子的生产，而不影响其生物学活性。
独特的生物学优势
All mammalian cells secrete IGF Binding Proteins (IGFBPs) which bind to and inhibit native IGF-I. The substitution of an arginine for glutamic acid at position three in LONG® R3 IGF-I, in conjunction with the 13 amino acid N-terminal extension peptide, results in > 1000 fold reduced affinity for IGFBPs, enhancing its bio-availability and effectiveness in comparison to native IGF-I.
特异性是提高产量的关键
Unlike insulin, LONG® R3 IGF-I specifically targets and activates the type I IGF Receptor (IGF-IR) resulting in greater activation of downstream signaling cascades responsible for proliferation and inhibiting apoptosis. Greater activation of these key pathways results in increased cell density, higher viability and extended cell culture productivity.
To learn more about the use of LONG® R3 IGF-I in stem cell and cell therapy applications, please contact Repligen.
优化细胞培养
产量
Cell culture supplements are essential for long-term growth and productivity of cell lines in serum-free media formulations. Both insulin and LONG® R3 IGF-I, a more potent supplement that directly targets and activates the IGF-I receptor, are employed by the biopharmaceutical industry today to support the growth of recombinant cell lines. This webinar will show how these two supplements, used separately and in combination, impact viable cell density and IgG production of multiple CHO cell lines. It will demonstrate how a combination of LONG® R3 IGF-I and insulin results in less protein supplementation and, therefore, less potential impact on downstream processing.
HIGH PERFORMANCE
Improved volumetric productivity
LONG® R3 IGF-I has been shown to improve volumetric productivity in CHO cell culture by up to 62% over cell culture with no supplementation and 40% over cell culture supplemented with insulin.
LONG® R3 IGF-I Cell Culture Supplement
LONG® R3 IGF-I is a critically important mitogenic growth factor often used in mammalian cell culture to increase yield significantly. Such growth factors are fundamental supplements to chemically-defined media.
LONG® R3 IGF-I is compatible with multiple cell types. Any mammalian cell type that responds to insulin in cell culture has the potential to respond to LONG® R3 IGF-I.
- CHO 克隆
- HEK 293
- Vero
- MDCK
- 成纤维细胞
LONG® R3 IGF-I is available in two formats, both stable at 2-8°C.
LONG® R3 IGF-I can also be milled into cell culture media for additional flexibility and convenience.
Since LONG® R3 IGF-I ships at ambient conditions and stored at 2-8°C, cold chain shipping and storage costs can be significantly reduced.
冻干粉
- 5 mg (样品规格*)
- 50 mg
即用型液体
- 5 mL (样品规格*)
- 100 mL
- 250 mL
- 500 mL
- 1L (1000 mL)
*样品规格未完全验证。该物料使用经验证的中间体物料进行灌装。稳定性信息分别参考100mL和50mg稳定性试验。
LONG® R3 IGF-I ELISA Kit
The only commercially available kit for the quantitation of LONG® R3 IGF-I, the kit is fast, accurate, and easy to use with both cell culture and drug substance samples. The sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
- 对天然或重组IGF-1有限的反应性
- 对胰岛素无反应性
- 即用型ELISA板，可允许立即进行分析，而不需要额外的包板及封闭步骤
试剂盒包括：
- LONG® R3 IGF-I coated strip plate, 96 wells
- LONG® R3 IGF-I Standard
- 稀释液
- 生物素化检测抗体
- 链霉亲和素过氧化物酶
- TMB过氧化物酶底物
- LoBind® 1.5mL EP管
客户至上。
Repligen DNA 为您提供支持。我们的目标是提供卓越的客户体验，并为所有 Repligen 产品和服务高效成功的采用及实施提供支持。
- Field Application Support
- 客户服务
- 现场服务工程师
Related products
LONG R3 IGF-I ELISA Kit
The LONG® R3 IGF-I ELISA Kit is the only commercially available kit for the quantitation of LONG® R3 IGF-I. Fast, accurate, and easy to use with both cell culture and drug substance samples, this sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
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