TangenX® Flat Sheet Membranes

TangenX^® 平板膜包使用两种改性聚醚砜(mPES)膜材质，ProStream 和 HyStream，其可为不同的应用提供出色的选择性。

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高性能

均匀的膜孔分布

高选择性

高通量

合规

GMP-validated ProStream and Hystream

HIGH PERFORMANCE

Uniform Pore Distribution

超滤膜的选择性在于表面层，也称为膜的“皮层”。皮层的下层由“指状孔”结构组成，可支撑皮层，并促进液体流动。

The scanning electron micrograph image shows uniformity in the finger pores of the TangenX^® membrane substructure. This allows for minimal particle entrapment, which results in low fouling and maximum fluid flow. Repligen has developed this membrane substructure to ensure a defect-free surface and to provide maximum adhesion to the substrate while achieving highest possible flow rates.

Porous substructure of the TangenX^® ProStream 10K membrane

HIGH PERFORMANCE

高选择性

超滤膜使用标称截留分子量作为膜表面孔径分布的衡量标准。孔径更均一，则膜的选择性更为出色。更窄的孔径分布意味着更高的膜选择性；即膜有能力区分粒径更为接近的大分子。

图中曲线所示为Repligen提供的多种不同截留分子量规格膜的选择性特性。每种膜的选择性曲线与其它膜显著分开，没有重叠，说明其具有高度的选择性。这有利于获得更高的工艺性能和产物收率。

COMPLIANT

GMP-validated membranes

Repligen 生产两种用于平板膜包的膜，ProStream 和 HyStream，两者均提供超滤和微滤孔径规格。两者均为改性聚醚砜（mPES）膜，并经验证，可用于cGMP工艺。通过向浇铸前膜溶液中添加第二种聚合物，以实现修饰，可确保全面且一致的表面修饰。

ProStream membrane is neutrally charged, rendering it low binding for enhanced flux and recovery. HyStream membrane is extremely hydrophilic in nature, resisting fouling from hydrophobic specied such as fat, lipids, and various antifoam agents.

TangenX® Flat Sheet Membranes

TangenX flat sheet membranes achieve high flux and selectivity using a controlled manufacturing process that enables tight control of the membrane pore size. The process ensures a controlled transition from the selective surface to the microporous support.

Click on the tabs below to explore different features of the TangenX flat sheet membranes.

ProStream膜带中性电荷，所以结合较低，可获得更高的通量和收率。此类膜具有出色的化学耐受性和极低的溶出性，性能与Millipore Biomax、Pall Omega相当。

中性平衡电荷/低蛋白质结合
是高产物收率至关重要的下游纯化工艺的理想选择
经修饰，可降低由于非特异性结合而导致的蛋白质吸附
工艺通量和产物收率高于未经修饰的PES膜

截留分子量	SIUS® Single-use	PRO 重复使用
Ultrafiltration 1kD, 3kD, 5kD, 10kD, 30kD, 50kD, 100kD, 300kD	x	x

Since it is neutrally charged, ProStream membrane minimizes non-specific protein binding. Unmodified PES has an inherent negative charge and will have non-specific binding.

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HyStream membrane is extremely hydrophilic in nature, resisting fouling from hydrophobic species such as fat, lipids, and various antifoam agents. The membrane has good chemical resistance and offers an alternative to RC membranes in some cases, with performance comparable to Millipore Ultracel, and Sartorius Hydrosart.

亲水性 - 疏水性分子污染最小化 / 低蛋白质结合
是要求低污染、高滤液通量的上、下游过滤操作的理想选择
避免疏水性物质存在时经常发生的通量急剧衰减的情况

截留分子量	SIUS® Single-use	PRO 重复使用
Ultrafiltration 5kD, 10kD, 30kD, 50kD, 100kD, 300kD	x	x
Microfiltration 0.1µm, 0.2µm, 0.45µm, and 0.65µm	x	x

由于HyStream膜经修饰高度亲水，所以接触角低于中性电荷膜。

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Membrane Selection Guide

There are other factors that can influence the selectivity and retentivity of a membrane. The shape of a molecule, presence of other solutes, pressure, adsorption and ionic conditions may affect membrane performance. A Repligen Field Application Specialist can help guide membrane selection for specific applications.The following table shows typical use of flat sheet membrane chemistries by application.

In general, select a Molecular Weight Cut-off (MWCO) that is 3 to 6 times smaller than the protein or other molecule you want to retain
如首要任务是尽可能提高通量，选择更加开放的MWCO膜，即范围的下限(3X)
如主要目的是截留，选择更加致密的MWCO膜，即范围的上限(6X)

	应用	工艺	特点	优势
超滤	含有生物分子(白蛋白、单抗、IgG、IgM、激素、生长因子等)的蛋白质溶液的处理，	Concentration Buffer exchange Depyrogenation Membrane purification	高通量高选择性无缝隙低蛋白质结合高化学耐受性	更低的膜面积更小的系统高收率简单、快速的清洗出色的可再生性
微滤	处理细胞培养发酵液	收获澄清浓缩	高通量高选择性无缝隙低蛋白质结合高化学耐受性	更低的膜面积更小的系统高收率简单、快速的清洗出色的可再生性

膜片

Membrane discs are available using HyStream or ProStream in all porosities. TangenX^® membrane cut discs are available in common diameters to fit conventional stirred cells and the TangenX^® X-Flo76 membrane screening system. Discs are disposable; however, if appropriate cleaning measures are taken, they may be washed and reused typically 10 to 20 times. Disc sizes are available in 25 to 90 mm.

下表为可提供的膜片尺寸列表。订购时，请确定膜类型和截留分子量。

膜片尺寸	数量	产品编号
25 mm圆片	12 片	D025
44 mm圆片	12 片	D044
47 mm圆片	12 片	D047
63 mm圆片	12 片	D063
76 mm圆片	12 片	D076
90 mm圆片	12 片	D090
203 mm x 228 mm 方片	1 片	SR01
203 mm x 228 mm 方片	5 片	SR05

TFF genius

You’re not asking for too much. Combine high selectivity and reduced processing time. Become a TFF genius and get it all with TangenX® Membranes and TFF Devices.

RGEN

Manufacturing Centers of Excellence

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品，以确保我们客户的业务连续性。

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.

地点

经验证的生产过程

高质量生产

TangenX^® Cassettes are manufactured in a fully validated and documented manufacturing process according to the principles of cGMP. Each cassette comes with a Quality Assurance Certificate.