OPUS RoboColumn Pre-packed Chromatography Colums
Used in the industry since 2007, OPUS RoboColumn pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.
Up to 96 RoboColumn pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.
![96 well plate OPUS RoboColumn pre-packed chromatography column](https://www.repligen.com/Products/OPUS/OPUS%20RoboColumn_Plate_Intro_Start_Configurator_Shopping%20card%20%282%29.png)
OPUS® RoboColumn®
internal design
OPUS® RoboColumn®
5 mm ID x 10 mm H; V = 200 µL
OPUS® RoboColumn®
5 mm ID x 30 mm H; V = 600 µL
OPUS® RoboColumn®
96-well plate
![](https://www.repligen.com/Products/OPUS/OPUS_Lineup_13Feb2024.jpg)
Click and quote OPUS® PD Pre-packed chromatography columns
![homescreen of OPUS robocolumn configurator](https://www.repligen.com/_default_upload_bucket/get-image-thumbnail_51.png)
Configure and quote pre-packed columns for process development using this interactive tool.
When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes.
Make your chromatography life easier.
FLEXIBILITY
Flexibility with pre-packed convenience
- 可预装用户指定的层析填料 - 已超300种填料类型
- Consistently packed with correct axial compression
- 以液体处理工作站的移液速度控制的线性流速操作
- 由于恒定的保留时间,具有经证实的规模放大和规模缩小性能
- 单位时间更多的样品数量,多重平行开发的能力
可选构型
内径 |
柱床高度 |
柱体积 |
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cm | mm | cm | mm | mL | µL |
0.5 | 5.0 | 0.25 | 2.5 | 0.05 | 50 |
0.5 | 5.0 | 0.50 | 5.0 | 0.10 | 100 |
0.5 | 5.0 | 1.00 | 10.0 | 0.20 | 200 |
0.5 | 5.0 | 2.25 | 22.5 | 0.45 | 450 |
0.5 | 5.0 | 2.50 | 25.0 | 0.50 | 500 |
0.5 | 5.0 | 3.00 | 30.0 | 0.60 | 600 |
Minimum order is one row of 8 OPUS RoboColumn pre-packed columns with the same resin and column dimension.
自动化
Automated parallel chromatography
Shorten process development time by performing parallel operation of OPUS RoboColumn pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design). The combination of OPUS RoboColumn and Tecan robotic liquid handling workstations deliver a complete integrated platform for state of the art small scale protein purification.
Designed for robotic fluid handlers
Compatitble with both Tecan Fluent and Freedom EVO liquid handling workstations
- 同时自动步骤-梯度洗脱至96孔板中
- 在集成的读板器中进行后续的分析
- 灵活的软件向导
![](https://www.repligen.com/Products/OPUS/Fluent_780_Te-Chrome_V1_01%201%20%282%29.png)
Rapid and simple AAV capture and polish DOE with new digital tools
This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan Fluent liquid handling workstation.
![视频海报](https://img.youtube.com/vi/qDtpvn2rIb8/0.jpg)
High Performance
工艺开发![](https://www.repligen.com/Products/OPUS/OPUS_Diagram01 (2).jpg)
Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step.
- Resin screening
- Condition screening
Using OPUS RoboColumn on a Tecan Fluent liquid handling workstation generated up to 96 pseudo-chromatograms (1536 data-points) in less than one day.
样品制备 / 过程分析技术 (PAT)
The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples derived from small scale multi-parallel bioreactors, such as Ambr™, requires sample preparation before analysis can be executed.
- Drug discovery and clone selection
- 蛋白质表征
- Media optimization
- 发酵过程中监测
Using the OPUS RoboColumn on a Tecan Fluent liquid handling workstation generates sufficient purified product to conduct your analytical assays.
![](https://www.repligen.com/Pictures/4043/image-thumb__4043__width1920/center-of-excellence-collage.jpg)
![](https://www.repligen.com/_default_upload_bucket/3376/image-thumb__3376__width536/alex-support-md.jpg)
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- Field Application Support
- 客户服务
- 现场服务工程师
规格
板尺寸 |
128.3 L x 86.0 W x 14.0 mm H |
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板材料 |
聚氧亚甲基共聚物 |
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孔数 |
96 |
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柱体积 |
50 µL |
100 µL |
200 µL |
450 µL |
500 µL |
600 µL |
柱床高度 |
2.5 mm |
5.0 mm |
10.0 mm |
22.5 mm |
25.0 mm |
30.0 mm |
层析柱内径 直径 |
5 mm |
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柱材料 |
聚丙烯 |
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化学稳定性 |
所有常用水性缓冲液,pH 1 – 14,有机溶剂*。 |
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柱储存溶液 |
AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride |
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推荐 储存温度 |
AIEC、CIEC、HIC、SEC 和 MMC:+4°C - +30°C |
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工作温度 |
+4°C - +30°C |
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最高工作 压力 |
最高 8.0 bar |
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流速 |
16 – 1000 cm/h** |
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标签 |
激光印刻在每根层析柱柱体上。标签包含:
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* 注意:还需要考虑所预装的层析填料的化学稳定性。更多信息,请参考厂商的填料规格说明。