OPUS RoboColumn Pre-packed Chromatography Colums

Used in the industry since 2007, OPUS RoboColumn pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.

Up to 96 RoboColumn pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.

96 well plate OPUS RoboColumn pre-packed chromatography column

OPUS® RoboColumn®
internal design


OPUS® RoboColumn®
5 mm ID x 10 mm H; V = 200 µL

OPUS® RoboColumn®
5 mm ID x 30 mm H; V = 600 µL

OPUS® RoboColumn®
96-well plate

Click and quote OPUS® PD Pre-packed chromatography columns


homescreen of OPUS robocolumn configurator

Configure and quote pre-packed columns for process development using this interactive tool.

When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes. 

Make your chromatography life easier.

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Flexibility with pre-packed convenience

  • 可预装用户指定的层析填料 - 已超300种填料类型
  • Consistently packed with correct axial compression
  • 以液体处理工作站的移液速度控制的线性流速操作
  • 由于恒定的保留时间,具有经证实的规模放大和规模缩小性能
  • 单位时间更多的样品数量,多重平行开发的能力





cm mm cm mm mL µL
0.5 5.0 0.25 2.5 0.05 50
0.5 5.0 0.50 5.0 0.10 100
0.5 5.0 1.00 10.0 0.20 200
0.5 5.0 2.25 22.5 0.45 450
0.5 5.0 2.50 25.0 0.50 500
0.5 5.0 3.00 30.0 0.60 600

Minimum order is one row of 8 OPUS RoboColumn pre-packed columns with the same resin and column dimension.


Automated parallel chromatography

Shorten process development time by performing parallel operation of OPUS RoboColumn pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design). The combination of OPUS RoboColumn and Tecan robotic liquid handling workstations deliver a complete integrated platform for state of the art small scale protein purification.

Designed for robotic fluid handlers 

Compatitble with both Tecan Fluent and Freedom EVO liquid handling workstations

  • 同时自动步骤-梯度洗脱至96孔板中
  • 在集成的读板器中进行后续的分析
  • 灵活的软件向导 

Rapid and simple AAV capture and polish DOE with new digital tools

This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan Fluent liquid handling workstation.


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 High Performance


Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step.

  • Resin screening
  • Condition screening

Using OPUS RoboColumn on a Tecan Fluent liquid handling workstation generated up to 96 pseudo-chromatograms (1536 data-points) in less than one day.

样品制备 / 过程分析技术 (PAT)


The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples derived from small scale multi-parallel bioreactors, such as Ambr™, requires sample preparation before analysis can be executed.

  • Drug discovery and clone selection 
  • 蛋白质表征
  • Media optimization
  • 发酵过程中监测

Using the OPUS RoboColumn on a Tecan Fluent liquid handling workstation generates sufficient purified product to conduct your analytical assays.

Manufacturing Centers of Excellence

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品,以确保我们客户的业务连续性。

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.



Repligen DNA 为您提供支持。我们的目标是提供卓越的客户体验,并为所有 Repligen 产品和服务高效成功的采用及实施提供支持。

  • Field Application Support
  • 客户服务
  • 现场服务工程师





128.3 L x 86.0 W x 14.0 mm H






50 µL

100 µL

200 µL

450 µL

500 µL

600 µL


2.5 mm

5.0 mm

10.0 mm

22.5 mm

25.0 mm

30.0 mm

层析柱内径 直径

5 mm




所有常用水性缓冲液,pH 1 – 14,有机溶剂*。


AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride
HIC, SEC, Affinity and MMC Columns: 20 % ethanol
CHT, CFT Columns (Bio-Rad): 20 % ethanol + 10 mM sodium phosphate, pH 6.8

推荐 储存温度

亲和层析柱:+2°C - +8°C


+4°C - +30°C

最高工作 压力

最高 8.0 bar


16 – 1000 cm/h**



  • 产品编号
  • 填料名称
  • 层析柱体积(µL)

* 注意:还需要考虑所预装的层析填料的化学稳定性。更多信息,请参考厂商的填料规格说明。
** TECAN software enables set up of the flow rate for each individual dispensing and chromatography step.