ELISA 试剂盒
ELISA Kits from Repligen deliver the precision, reproducibility, and sensitivity required for the accurate quantitation of leached native Protein A, recombinant Protein A, the MabSelect SuRe™ ligand, the NGL-Impact® A ligand, the NGL COVID-19 Spike Protein Affinity Ligand, and LONG® R3 IGF-I.
REPRODUCIBLE
ACCURATE
Protein A 检测
Detection of leached Protein A at low limits is critical for accurate testing and reliable results. The Protein A ELISA Kits from Repligen deliver consistent recovery performance throughout the detection range of the assay across different process buffers.
![graph showing recovery of leached Protein A ligand](https://www.repligen.com/Products/ELISA/3880/image-thumb__3880__width536/2dfd1feba2b051e937cbd55327f5074d.jpg)
![graph showing recovery of leached MabSelect SuRe ligand](https://www.repligen.com/Products/ELISA/3881/image-thumb__3881__width548/ELISA_detection.jpg)
ACCURATE
Protein A Quantitation
尽管FDA没有发布针对脱落Protein A 可允许水平的说明,但是生产商必需对最终治疗性制剂中的Protein A污染物水平进行检测和定量。
Repligen提供的Protein A试剂盒由于其高分析回收率和灵敏度,已被多种商品化/临床阶段单克隆抗体和Fc融合蛋白治疗药物生产商用于产品质量以及工艺污染物的出厂检测。
![](https://www.repligen.com/Products/ELISA/Protein_A_quantitation.jpg)
REPRODUCIBLE
可重复性
Repligen提供的Protein A ELISA试剂盒经证实具有重要的试剂盒间和试剂盒内一致性。可重复性见下表,针对重复样品及3种模式缓冲液,在分析的动态范围内具有高度精确性(%CV)。
![](https://www.repligen.com/Products/ELISA/3883/image-thumb__3883__width424/d138f636764d00f6d5f397697015f79e.jpg)
![](https://www.repligen.com/Products/ELISA/3884/image-thumb__3884__width424/041bbbe220442b1519460e9a48367714.jpg)
The data above were acquired using the Dilute & Go method. In all cases the sample buffer/hIgG matrix was tested in triplicate at 6 different Protein A concentration levels in three independent assays. The precision (inter and intra assay) was determined for both the Recombinant Protein A and MabSelect SuRe™ kits. The common buffers used were100 mM Glycine, 100 mM Acetate, and 100 mM Citrate. All buffers were prepared at a pH of 3.0 and neutralized to pH 7.6 with Tris-base. Each contained 5 mg/mL of human polyclonal IgG.
OPTIMIZED PROTOCOLS
样品制备方案
Repligen设计了3种样品制备方案,以最大限度地优化使用的简便性、速度以及检测和定量的限值。
Dilute and Go™(稀释和运行)
- 最大限度提高使用的简便性,节省分析运行时间,无需缓冲液置换步骤
- 样品可直接稀释进行分析*
Boil and Boost™(煮沸和增强)
- 提高样品输入浓度,并降低定量限值
- Combines the low pH dissociation step with boiling (no buffer exchange step**)
原始方案
- 适用于传统方法
- 需要缓冲液置换步骤
*必需评估特异性基质,以确保适用性
**在Boil & Boost™方案中,某些缓冲液可能需要透析
![photo of a scientist running the ELISA assay kit in a lab hood.](https://www.repligen.com/Products/ELISA/3b598ca7db7ef67204e88072b34e7070.jpg)
ELISA 试剂盒
Click on the tabs to explore features of the different ELISA Kits by target ligand.
对于使用MabSelect SuRe™ 捕获步骤的下游工艺,Repligen提供市面上唯一含有SuRe™配基标准品的试剂盒,以进行最准确的定量。
- 准确匹配的Protein A 参考标准品(SuRe™配基)
- 灵敏度0.1 ng/mL
- 回收率:80-120%
- 准确性 & 精确性:试剂盒间分析 +/- 10%,试剂盒内分析 +/- 15%
试剂盒包括:
- 抗-protein A 包被的96孔板
- 试剂A:样品稀释液(5X)
- 试剂B:MabSelect SuRe™ 标准品溶液
- 试剂C:兔抗-protein A:生物素探针
- 试剂D:链霉亲和素:过氧化物酶偶联物
- 试剂E:TMB过氧化物酶底物
- PBS包(数量2)
![](https://www.repligen.com/Products/ELISA/ELISA_ELISA_MabSelect_SuRe_Kit_Lo_Res_021918.png)
For downstream processes that utilize a Praesto® Jetted A50 capture step, Repligen offers the only kit on the market that includes the NGL-Impact® A Ligand standard for the most accurate quantitation.
- Exact matched Protein A reference standard (NGL-Impact® A Ligand)
- 灵敏度0.1 ng/mL
- 回收率:80-120%
- 准确性 & 精确性:试剂盒间分析 +/- 10%,试剂盒内分析 +/- 15%
试剂盒包括:
- 抗-protein A 包被的96孔板
- 试剂A:样品稀释液(5X)
- Reagent B: NGL-Impact® A standard solution
- 试剂C:兔抗-protein A:生物素探针
- 试剂D:链霉亲和素:过氧化物酶偶联物
- 试剂E:TMB过氧化物酶底物
- PBS包(数量2)
![](https://www.repligen.com/Products/ELISA/ELISA_ELISA_MabSelect_SuRe_Kit_Lo_Res_021918.png)
The LONG® R3 IGF-I ELISA Kit is the only commercially available kit for the quantitation of LONG® R3 IGF-I. Fast, accurate, and easy to use with both cell culture and drug substance samples, this sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
- 对天然或重组IGF-1有限的反应性
- 对胰岛素无反应性
- 即用型ELISA板,可允许立即进行分析,而不需要额外的包板及封闭步骤
试剂盒包括:
- LONG® R3 IGF-I coated strip plate, 96 wells
- LONG® R3 IGF-I Standard
- 稀释液
- 生物素化检测抗体
- 链霉亲和素过氧化物酶
- TMB过氧化物酶底物
- LoBind® 1.5mL EP管
![](https://www.repligen.com/Products/ELISA/elisa-kit-long-r3-igf.png)
For downstream processes that utilize a Praesto® Jetted A50 Hi pH capture step, Repligen offers the only kit on the market that includes the NGL-Impact® A Hi pH Ligand standard for the most accurate quantitation.
- Exact matched Protein A reference standard (NGL-Impact® A Hi pH Ligand)
- 灵敏度0.1 ng/mL
- 回收率:80-120%
- 准确性 & 精确性:试剂盒间分析 +/- 10%,试剂盒内分析 +/- 15%
试剂盒包括:
- 抗-protein A 包被的96孔板
- 试剂A:样品稀释液(5X)
- Reagent B: NGL-Impact® A Hi pH Ligand standard
- 试剂C:兔抗-protein A:生物素探针
- 试剂D:链霉亲和素:过氧化物酶偶联物
- 试剂E:TMB过氧化物酶底物
- PBS包(数量2)
![](https://www.repligen.com/Products/ELISA/9777-1_ELISA_NGL_ImpactA_HipH_Kit.png)
对于使用Protein A捕获步骤(非MabSelect SuRe™)的下游工艺,Repligen提供对残留的天然和重组Protein A (rPA)具有广泛识别性的Protein A ELISA试剂盒。
- 针对所有天然和重组Protein A异构体的特异性
- 灵敏度0.1 ng/mL或0.8 ppm
- 回收率:80-120%
- 准确性和精确性:试剂盒间+/-10%,试剂盒内+/-15%
试剂盒包括:
- 抗-protein A 包被的96孔板
- 试剂A:样品稀释液(5X)
- 试剂B:重组Protein A标准品溶液
- 试剂C:兔抗-protein A:生物素探针
- 试剂D:链霉亲和素:过氧化物酶偶联物
- 试剂E:TMB过氧化物酶底物
- PBS包(数量2)
![](https://www.repligen.com/Products/ELISA/ELISA_rProtein_A_Kit-Lo_Res_021918%20copy.png)
![](https://www.repligen.com/Pictures/4043/image-thumb__4043__width1920/center-of-excellence-collage.jpg)
![](https://www.repligen.com/_default_upload_bucket/3376/image-thumb__3376__width536/alex-support-md.jpg)
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