KrosFlo® KMPi TFF System
Fully automate 100 ml - 500 L UF/DF processes using an unparalleled combination of hardware integration with peristaltic pump, configurability and automation through modular, plug-and-play hardware.
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integrated
Modular, plug-and-play hardware with peristaltic pump
The KrosFlo® KMPi TFF System achieves an unparalleled combination of integration, configurability and automation through modular, plug-and-play hardware. All components, either directly or indirectly, connect to the main KrosFlo® KMPi peristaltic pump, which then communicates with the KF Comm 2 software for control and monitoring.
- 可针对简单的应用，如浓缩（C）或洗滤（D），配置最低数量的部件
- Plug-and-play additional components for fractionation (F) and multi-step processes (i.e. CDC/D/C)
Click on the tabs below to explore different combinations of concentration and diafiltration configurations using the KrosFlo® KMPi System.
Concentrate with a streamlined configuration
C = Concentration D = Diafiltration F = Feed
Add a scale and auxiliary pump to access diafiltration applications
C = Concentration D = Diafiltration F = Feed
Build process understanding with increased sensors
C = Concentration D = Diafiltration F = Feed
integrated
ProConnex® Flow Paths for KMPi
Save time, streamline operations and hit the ground running
These ready-to-go flow paths create time and quality gains for every run. Enjoy the affordable convenience of off-the-shelf flow paths designed by the engineers who created the system you adore. These are created specifically to run Spectrum® Hollow Fiber or TangenX® flat sheet cassettes on the KrosFlo KMPi systems. Filter sold separately.
integrated
通过专利主板，
双向传输数据
An advanced microprocessor-based controller is integrated into the main KrosFlo® KMPi System pumps, enabling plug-and-play hardware component behavior. Bi-directional communication is established between the KF Comm 2 software and hardware components, such as the Automatic Back Pressure Control Valve, scales, auxiliary pumps and the Konduit device.
KrosFlo KR2i Virtual Demo Series
In this 6-part series, Julie Grace, and Carl Breuning, provide viewers with a virtual training on the KrosFlo® KR2i TFF system. This training series will cover the following topics:
- Introduction to system components
- Installation of flow-path and filter
- KF Comm2 software overview
- Recipe creation
- Running a CFC/D/C experiment
- Recovery of product
KrosFlo® KR2i Virtual Demo Series
In this 6-part series, Julie Grace, and Carl Breuning, provide viewers with a virtual training on the KrosFlo® KR2i TFF system. This training series will cover the following topics:
- Introduction to system components
- Installation of flow-path and filter
- KF Comm2 software overview
- Recipe creation
- Running a CFC/D/C experiment
- Recovery of product
AUTOMATED
Menu-driven Software for Optimized TFF
KF Comm 2 Software, backward compatible with legacy KrosFlo® KR2i and KrosFlo® KMPi Systems, is conveniently installed on any computer using the Windows Operating System. The software is compatible with Chrome, Mozilla, Firefox and Microsoft Explorer browsers and is always current with automatic software and firmware updates.
- 用于KrosFlo® TFF 系统的专利软件
- 提供21CFR Part 11合规版本，以验证用于GMP工艺
- 直观的工作流，13种操作模式
AUTOMATED
自动背压控制阀
Repligen独家技术，自动背压控制阀为每个TFF工艺增加跨膜压（TMP）控制，减少操作员手动操作时间，提高工艺可再现性。
- 按用户设置的设定点，自动调整管夹距离
- 在整个工艺运行过程中，维持均一的TMP，降低膜污染
- 在微滤应用中，控制滤液压力和流速
- 使用兼容管号：13、 14、 16、 25、 HP15、 17、 HP24、 18、 HP35、 36 和 HP36
- 可用于自动调节进样、回流、滤液或跨膜压力
AUTOMATED
Monitor and evaluate conductivity, UV and temperature
Konduit is an add-on module ready to use with the KrosFlo® KR2i and KMPi TFF Systems, with the ability to scale from 2mL to 500L process volumes.
READY TO SCALE FAST
Ideal for Gene Therapy from Lab to Manufacturing
Run TFF development experiments and compliant small-scale manufacturing runs on a single platform. Start with the KrosFlo® KR2i System with volumes as low as 10 mL. Scale up to 500 L with the KrosFlo® KMPi System while maintaining pump design, system architecture and software.
- 在同一平台内进行规模放大和规模缩小
- 针对不同应用进行工艺序列配置（C、D和CFC/D/C）
- Modern 21 CFR Part 11 compliance software built on secure Microsoft® SQL Server
READY TO SCALE FAST
Scalable From Lab-scale to Process-scale
The KrosFlo® family of TFF Systems for concentration and diafiltration supports scale up from process development to commercial manufacturing.
KrosFlo® KMPi TFF System
The fully integrated KrosFlo® KMPi TFF System consists of hardware, software and flow path components. Key hardware components include: a digital peristaltic pump, auxiliary pumps and an automatic back pressure valve.
The KF Comm 2 software features a multi-language interface for complete monitoring and control and an optional 21 CFR Part 11 environment. Add tubing and sanitize/sterilize your own flow path around a Spectrum® hollow fiber filter or build a closed and gamma-irradiated ProConnex® Flow Path from over 250 components.
Click on the links below to explore different features.
解决方案
Optimized for use with Repligen Hollow Fiber Filter Modules
| 主要 应用 |
体积 | 进口 / 出口 | 侧面 端口 |
标尺 长度 |
有效长度 (cm) | 总 长度 (cm) |
表 面积 |
|
|---|---|---|---|---|---|---|---|---|
MiniKros Sampler |
||||||||
 |
小体积批量TFF | 3 L -15 L | 0.75" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 47 70 |
490 – 1000 cm2 1000 - 2200 cm2 1600 - 3400 cm2 |
MiniKros |
||||||||
 |
中试规模TFF | 5 L - 50 L | 1.5" TC | 0.75" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
25 46 70 |
0.16 - 0.31 m2 0.32 - 0.65 m2 0.51 - 1.0 m2 |
KrosFlo® |
||||||||
 |
生产规模TFF | 10 – 100L | 3" TC | 1.5" TC | 1 x FL 2 x FL 3 x FL |
20 41.5 65 |
23 46 69 |
0.78 - 1.6 m2 1.65 - 3.25 m2 2.55 - 5.10 m2 |
部件和配件
- IP 33 级外壳
- 600 RPM 泵，带可逆驱动
- 90-260 Vrms @ 50/60 Hz
- 标准 Easy Load 3 泵头，适用高精度13、14、16、25、17 和 18 号L/S管。
- (选配) Easy Load 3 泵头，适用高性能15、24、35、36号L/S管。
- ETL注册，带CE/UL/cUL标识
- 泵驱动尺寸：10.5 in x 8 in x 8 in
KONDUiT可增加：
- 用于UF/DF的终点电导控制
- 用于从药物递送颗粒中洗滤去除游离药物的终点UV控制
- 用于特定分子浓缩时的终点UV控制
- 用于膜穿透的UV警报
- 提供A260 和 A280 光源
*UV 部件单独销售
Expert Project Management
客户至上。
Repligen DNA 为您提供支持。我们的目标是提供卓越的客户体验，并为所有 Repligen 产品和服务高效成功的采用及实施提供支持。
- Field Application Support
- 客户服务
- 现场服务工程师
KrosFlo® KTF TFF Systems
The KrosFlo KTF are a series of highly configurable single-use TFF systems for multiple unit operations, applications, and filter types. With volumes spanning across 1 to 5000L, users enjoy the unrivaled flexibility of processing large and small batch sizes within the same platform.
KrosFlo® RS TFF Systems
The KrosFlo® RS are a series of tangential flow filtration (TFF) systems engineered for an era of new modalities. These systems are single-use and cGMP compliant with features like end-to-end automation, minimal hold-up volumes, and effortless scalability. Support automated ultrafiltration and diafiltration (UF/DF) processes for batch volume ranges of 35mL to 2000L.
ProConnex® Flow Paths for Systems
Expertly designed plug-and-play flow path
ProConnex® Flow Paths for TFF and Chromatography processes are single-use and engineered for optimum performance, reproducibility and time savings. System specific flow paths provide speed and reliability. Configured flow paths prioritize process requirements and sterility.
Process Analytics
Our process analytics solutions, featuring Variable Pathlength Spectroscopy for at-line and in-line concentration monitoring systems, ensure that products meet required Process Analytical Technology (PAT) standards. These tools provide critical data for optimizing bioprocesses and ensuring compliance with regulatory requirements.
资源
规格
|
Description |
规格 |
|---|---|
| 工艺体积 | 可达500L |
| 过滤器面积 | 最高 5.1 m2 |
| 主泵/进样泵 | 最高 11LPM (650 RPM) |
| 压力传感器 | 最多 3 个鲁尔端口类型、聚砜、一次性使用 (-0.48 - 5.2bar) |
| 天平 | 60Kg (已包括)；可选配： 60Kg、200Kg、500Kg 和 1000Kg |
| 辅助泵 | KrosFlo® Research I，600 RPM |
| 管路 | PharmaPure，TPE，管号 #26、#73 和 #82 |
| TMP 控制 | 自动背压控制阀 (非侵入式/无产品接触) |
| 容器 | 10L 聚丙烯，含 4-端口盖和底部排液 |
| 作为HMI的平板电脑 | Microsfot Surface Pro |
| 软件 | KF Comm 2，可选配21CFR part 11软件包 |
| 合规 | UL & CE 认证 |