KrosFlo® TFDF® 系统
颠覆性的 TFDF®（切向流深度过滤）技术可简化和强化上游病毒载体生物工艺，是 AAV、慢病毒、溶瘤病毒、逆转录病毒、腺病毒、外泌体和微载体上细胞的理想选择。
Intensification Starts Here!
Discover how KrosFlo® TFDF® based process intensification leads to high virus production combined with simplified clarification compared to batch cell culture.
- Implement TFDF® to mitigate cell density effects
- TFDF® facilitates intensified production of lentivirus and AAV
- Simplify clarification techniques to streamline your production process
INNOVATION BREAKTHROUGH
TF + DF = TFDF® Combined Benefits
切向流
高细胞密度
低产物通过
深层过滤
低细胞密度
出色的产物通过
切向流深层过滤
高细胞密度
出色的产物通过
INNOVATION BREAKTHROUGH
Novel TFDF® Filter
The thick-walled 2 - 5 µM tubular filter captures cells and cell debris with reduced fouling and negligible product retention. The feed stream enters the bottom port. The retentate travels through the lumen and exits at the top of the tube. The permeate exits the enclosure through the lower permeate port.solution. The combination of tangential flow and depth filtration in one filter produces a high-flux, high-capacity solution. h
INNOVATION BREAKTHROUGH
协同优势
- Low concentration polarization minimizes fouling
- Short, highly inter-connected paths do not retain product
- 占地小
- 2-5 micron pore size for large biomolecules
INTEGRATED
Filter, Flow Path and System as One Solution
KrosFlo® TFDF® Filter
ProConnex® TFDF® Filter Assembly
KrosFlo® TFDF® Systems
INTEGRATED
Complete, Ready-to-use TFDF® Filter Assemblies
Single-use, Gamma-irradiated and closed ProConnex® TFDF® Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state. ProConnex® TFDF® Flow Paths are complete assemblies that include the TFDF® Filter, a disposable pump head, integrated sensors, tubing and aseptic connectors.
TFDF® Filter
Surface Area: 3 cm2
Surface Area: 30 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 3 cm2
Surface Area: 30 cm2
Surface Area: 50 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 150 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 450 cm2
Surface Area: 750 cm2
Surface Area: 1500 cm2
Surface Area: 2100 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 6000 cm2
Industrial grade components and extended automation allow for confident process scaling. With the same software, hardware, controls and cGMP compliance built into every system, process change challenges in scale-up are removed.
READY TO SCALE FAST
Maintain processes while scaling to production
The KrosFlo® TFDF® System delivers application-specific process control logic for optimal TFDF process performance. Systems scale from 1 L to 2000 L in a small footprint of 1 m2 for both pilot (31 inch x 42 inch) and process (37 inch x 42 inch) systems.
KrosFlo® TFDF Lab System
1 - 50 L volume
3 - 160 cm2 surface area
KrosFlo® TFDF® Pilot System
1 - 500 L volume
500 - 1600 cm2 surface area
KrosFlo® TFDF® Process System
50 - 2000L volume
3000 - 6000 cm2 surface area
READY TO SCALE FAST
Capacity increases with tube number and length
为确保从1 L到2000 L的线性规模放大，组件内纤维管数和管长度随处理量而增加。管长度可从20 cm增加至108 cm。过滤器的有效长度可能低于过滤器的物理长度。单根组件内的管数可从1根增加至40根。
管数
1 根管道
表面积：3 cm2
推荐处理体积：<1 L
1 根管道
表面积：150 cm2
推荐处理体积：<50 L
10 根管道
表面积：1500 cm2
推荐处理体积：<500 L
40 根管道
表面积：6000 cm2
推荐处理体积：<2000 L
* additional sizes available
管长度
物理长度：20 cm
有效长度：2 cm
表面积：3 cm2
物理长度：108 cm
有效长度：108 cm
表面积：150 cm2
KrosFlo® TFDF® 系统
The KrosFlo® TFDF® System delivers a complete solution for optimized tangential flow depth filtration, with integrated process control logic and a single-use, Gamma-irradiated ProConnex® TFDF® Flow Path with integrated sensors.
Click on the tabs to explore features of the different KrosFlo® TFDF® Systems and Flow Paths.
KrosFlo® TFDF® 实验室系统
1 - 50L 体积
3 - 160 cm2 表面积
Click on the links to explore different features.
KrosFlo® TFDF® Pilot System
1 - 500L 体积
500 - 1600 cm2 表面积
Click on the links to explore different features.
KrosFlo® TFDF® Process System
50 - 2000L 体积
3000 - 6000 cm2 表面积
Click on the links to explore different features.
ProConnex® TFDF® Flow Path
Click on the links to explore different features.
KrosFlo TFDF Lab Flow Paths
Off-the shelf, single-use, gamma-irradiated and closed TFDF Lab Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.
Custom KrosFlo TFDF Pilot and Process Flow Paths
When scaling up, configure a flow path to meet your process requirements for your pilot and process KrosFlo TFDF Systems. All KrosFlo TFDF flow paths are single-use, gamma-irradiated and arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.
Replace sluggish messy depth filtration with combined tangential flow and depth filtration. No need to load multiple filters or hunt for parts. At 1L through 2000L, connect one fully integrated filter module and go -- at flux speeds up to 1950 LMH.
2020-12-14
01:35
If you have three minutes, then you have enough time to install a TFDF® flow path. Mount the device, make a few connections and you’re done - without ever breaking a sweat.
2021-02-25
01:57
Expert Project Management
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相关产品
ProConnex® Flow Paths for Systems
Expertly designed plug-and-play flow path
ProConnex® Flow Paths for TFF and Chromatography processes are single-use and engineered for optimum performance, reproducibility and time savings. System specific flow paths provide speed and reliability. Configured flow paths prioritize process requirements and sterility.
资源
Process intensification for lentiviral vector manufacturing using tangential flow depth filtration
by Robert M. Tona, Reeti Shah, Kimberly Middaugh, Justin Steve, João Marques, Blair R. Roszell, Cindy Jung
Cell Press, March 2, 2023
使用HEK-293生产细胞系和先进灌流工艺生产慢病毒载体
作者：Michelle Yen Tran 和 Amine A. Kamen
Frontiers, 7月 14 日, 2022
Lentiviral vector manufacturing process enchancement utilizing TFDF® technology
Thomas Williams、Oliver Goodyear、Lee Davies、Carol Knevelman、Michael Bransby、Kyriacos Mitrophanous & James Miskin
Cell and Gene Therapy Insights, 2020年4月
