Intensification Starts Here!

Discover how KrosFlo® TFDF® based process intensification leads to high virus production combined with simplified clarification compared to batch cell culture.

  • Implement TFDF® to mitigate cell density effects
  • TFDF® facilitates intensified production of lentivirus and AAV
  • Simplify clarification techniques to streamline your production process

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  INNOVATION BREAKTHROUGH

TF + DF = TFDF® Combined Benefits

切向流

高细胞密度
低产物通过

深层过滤

低细胞密度
出色的产物通过

切向流深层过滤

高细胞密度
出色的产物通过

  INNOVATION BREAKTHROUGH

Novel TFDF® Filter 

The thick-walled 2 - 5 µM tubular filter captures cells and cell debris with reduced fouling and negligible product retention. The feed stream enters the bottom port. The retentate travels through the lumen and exits at the top of the tube. The permeate exits the enclosure through the lower permeate port.​solution. The combination of tangential flow and depth filtration in one filter produces a high-flux, high-capacity solution.  h

  INNOVATION BREAKTHROUGH

协同优势

  • Low concentration polarization minimizes fouling
  • Short, highly inter-connected paths do not retain product​
  • 占地小
  • 2-5 micron pore size for large biomolecules
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  INTEGRATED

Filter, Flow Path and System as One Solution

KrosFlo® TFDF® Filter
ProConnex® TFDF® Filter Assembly
KrosFlo® TFDF® Systems
  INTEGRATED

Complete, Ready-to-use TFDF® Filter Assemblies

Single-use, Gamma-irradiated and closed ProConnex® TFDF® Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state. ProConnex® TFDF® Flow Paths are complete assemblies that include the TFDF® Filter, a disposable pump head​, integrated sensors​, tubing​ and aseptic connectors.

TFDF® Filter
Surface Area: 3 cm2
Surface Area: 30 cm2

ProConnex® TFDF®
Flow Path
Surface Area: 3 cm2
Surface Area: 30 cm2
Surface Area: 50 cm2

ProConnex® TFDF® 
Flow Path

Surface Area: 150 cm2

ProConnex® TFDF® 
Flow Path

Surface Area: 450 cm2
Surface Area: 750 cm2
Surface Area: 1500 cm2
Surface Area: 2100 cm2

ProConnex® TFDF®
Flow Path
Surface Area: 6000 cm2

Industrial grade components and extended automation allow for confident process scaling. With the same software, hardware, controls and cGMP compliance built into every system, process change challenges in scale-up are removed.

READY TO SCALE FAST

Maintain processes while scaling to production

The KrosFlo® TFDF® System delivers application-specific process control logic for optimal TFDF process performance. Systems scale from 1 L to 2000 L in a small footprint of 1 m2 for both pilot (31 inch x 42 inch) and process (37 inch x 42 inch) systems.

KrosFlo® TFDF Lab System

1 - 50 L volume
3 - 160 cm2 surface area

KrosFlo® TFDF® Pilot System

1 - 500 L volume
500 - 1600 cm2 surface area

KrosFlo® TFDF® Process System

50 - 2000L volume
3000 - 6000 cmsurface area

READY TO SCALE FAST

Capacity increases with tube number and length

为确保从1 L到2000 L的线性规模放大,组件内纤维管数和管长度随处理量而增加。管长度可从20 cm增加至108 cm。过滤器的有效长度可能低于过滤器的物理长度。单根组件内的管数可从1根增加至40根。

管数

1 根管道
表面积:3 cm2
推荐处理体积:<1 L

1 根管道
表面积:150 cm2
推荐处理体积:<50 L

10 根管道
表面积:1500 cm2
推荐处理体积:<500 L

40 根管道
表面积:6000 cm2
推荐处理体积:<2000 L

* additional sizes available 

 

管长度

物理长度:20 cm
有效长度:2 cm
表面积:3 cm2

物理长度:108 cm
有效长度:108 cm
表面积:150 cm2

KrosFlo® TFDF® 系统

The KrosFlo® TFDF® System delivers a complete solution for optimized tangential flow depth filtration, with integrated process control logic and a single-use, Gamma-irradiated ProConnex® TFDF® Flow Path with integrated sensors.

Click on the tabs to explore features of the different KrosFlo® TFDF® Systems and Flow Paths.

KrosFlo® TFDF® 实验室系统

1 - 50L 体积
3 - 160 cm2 表面积

Click on the links to explore different features.

KrosFlo® TFDF® Pilot System

1 - 500L 体积
500 - 1600 cm2 表面积

Click on the links to explore different features.

KrosFlo® TFDF® Process System

50 - 2000L 体积
3000 - 6000 cm2 表面积

Click on the links to explore different features.

ProConnex® TFDF® Flow Path

Click on the links to explore different features.

KrosFlo TFDF Lab Flow Paths

Off-the shelf, single-use, gamma-irradiated and closed TFDF Lab Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.

See specifications

More information

Custom KrosFlo TFDF Pilot and Process Flow Paths

When scaling up, configure a flow path to meet your process requirements for your pilot and process KrosFlo TFDF Systems. All KrosFlo TFDF flow paths are single-use, gamma-irradiated and arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.

See specifications

More information

R 因子视频博客
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Espresso depth filtration

Replace sluggish messy depth filtration with combined tangential flow and depth filtration. No need to load multiple filters or hunt for parts. At 1L through 2000L, connect one fully integrated filter module and go -- at flux speeds up to 1950 LMH.

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The 3-Minute drill

If you have three minutes, then you have enough time to install a TFDF® flow path. Mount the device, make a few connections and you’re done - without ever breaking a sweat.

Manufacturing Centers of Excellence

Repligen在ISO 9001质量管理体系下开发和生产用于生物制药行业的产品。我们专注于及时交付高质量、一致且稳健的产品,以确保我们客户的业务连续性。

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.

地点

Expert Project Management

客户至上。

Repligen DNA 为您提供支持。我们的目标是提供卓越的客户体验,并为所有 Repligen 产品和服务高效成功的采用及实施提供支持。

  • Field Application Support
  • 客户服务
  • 现场服务工程师

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资源

第三方参考文献精选

Process intensification for lentiviral vector manufacturing using tangential flow depth filtration
by Robert M. Tona, Reeti Shah, Kimberly Middaugh, Justin Steve, João Marques, Blair R. Roszell, Cindy Jung
Cell Press, March 2, 2023

使用HEK-293生产细胞系和先进灌流工艺生产慢病毒载体
作者:Michelle Yen Tran 和 Amine A. Kamen
Frontiers, 7月 14 日, 2022

Lentiviral vector manufacturing process enchancement utilizing TFDF® technology
Thomas Williams、Oliver Goodyear、Lee Davies、Carol Knevelman、Michael Bransby、Kyriacos Mitrophanous & James Miskin
Cell and Gene Therapy Insights, 2020年4月

View publication resources.