高产量收获
高产量收获 (HPH) 是 Repligen 开发的一种新的应用,可以完全无菌的方式收获补料分批生物反应器以便在捕获柱上处理,同时消除离心和深层过滤步骤。
![](https://www.repligen.com/Pictures/Laboratory/3407/image-thumb__3407__width278/hph.jpg)
High Productivity Harvest (HPH) uses an XCell ATF® Cell Retention Device after peak density is achieved in a typical Fed-Batch process. Fresh media is added in a set flow rate while the harvesting of the product is initiated. A closed single step process using a 0.2 µm filter, HPH can generate the same product titer in half the time or twice the product compared to traditional Fed-Batch at no additional time.
Whether your intended application is mAb, rProteins, lentivirus, enzymes, or vaccines, HPH can boost productivity and deliver more product, faster.
![](https://www.repligen.com/solutions/high-productivity-harvest/advantages-HPH.png)
Repligen solutions help overcome key challenges in High Productivity Harvest, with hands-on process and implementation consultation from global Field Applications Specialists.
High Productivity Harvest Workflow
Click on the workflow link to find productivity and throughput solutions you can implement today.
![](https://www.repligen.com/solutions/high-productivity-harvest/16cc28b50ffefdd603a0283a2115060e.jpg)
提高产率
- Generate up to 2X increase in protein production without additional time, OR
- 可在更短的天数收获相同的滴度,提高产率
Improve processes
- 获得更健康的培养,同时降低工艺相关杂质
- 降低单元操作和原材料数量
- Transition to a single-step 0.2 µm filtered, closed system
- 收获料液可直接用于下游纯化
Maintain a Fed-Batch process
HPH increases productivity and throughput while maintaining a fed batch process. No perfusion or media exchange of the N bioreactor is required, allowing the N bioreactor to maintain a single and discreet fed batch harvest point.
HPH yielded 5X more VCD
HPH achieved 20 million viable cell density (VCD) compared to 4 million for Fed-Batch. HPH viability was maintained above 100% over a period of 8 days. Fed-Batch viability began to drop after day 3 to approximately 75% at days 8-9. Higher VCD and viability translated to an increase in total product yield. HPH yielded approximately 1500 mg/day while the Fed-Batch process achieved 800-1000 mg/day in approximately 8-10 days.
![](https://www.repligen.com/solutions/high-productivity-harvest/db414fd3ca8a1a4f31ddde70ef58b799.png)
Higher VCD and viability with HPH
- 相比标准补料分批,使用HPH补料分批可使活细胞密度提高4倍
- 活性维持接近100%
![](https://www.repligen.com/solutions/high-productivity-harvest/hph_titer.png)
Higher product titer with HPH
- 相比传统补料分批,产物提高2倍
- 在与补料分批相同(或更少)的天数内收获
High Productivity Harvest Webinar
The webinar demonstrates how HPH works, presents initial results of up to a two-fold increase in protein production, and illustrates how you can apply HPH specific to your product need or facility design.
![视频海报](https://img.youtube.com/vi/fmWY1lN6o7g/0.jpg)
工作原理
以下为HPH工艺操作的典型示例,其包括三个主要步骤:开始补料分批工艺,连接细胞截留装置开始高产量收获,之后开始抽液降低生物反应器内体积。HPH的启动时间是灵活的,且取决于工艺。收获可以在与传统补料分批相同的时间框架内完成,或者,生物反应器运行可以根据工艺和设施条件适当延长,以进一步提高产量。
步骤A:工艺开始
正常开始补料分批工艺。
步骤B:连接细胞截留设备(CRD),开始高产量收获
当达到峰细胞密度后,连接细胞截留装置(CRD),并开始以较低的流速泵入新鲜的培养基。随着新鲜培养基的泵入,开始慢慢收获产物。
步骤C:抽液
运行的最后一天,停止添加新鲜培养基,开始降低生物反应器内的体积。
步骤D:工艺结束
HPH补料分批可以在与传统补料分批相同的时间内结束,或延长生产运行,以进一步提高产量。
![](https://www.repligen.com/solutions/high-productivity-harvest/4bd5853e7726d607a75573f4157695cc.png)
注意:在本示例中,培养基置换在第10天开始,但也可根据工艺情况进行调节。
![](https://www.repligen.com/_default_upload_bucket/3376/image-thumb__3376__width536/alex-support-md.jpg)
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