高产量收获 (HPH) 是 Repligen 开发的一种新的应用，可以完全无菌的方式收获补料分批生物反应器以便在捕获柱上处理，同时消除离心和深层过滤步骤。
High Productivity Harvest (HPH) uses an XCell ATF® Cell Retention Device after peak density is achieved in a typical Fed-Batch process. Fresh media is added in a set flow rate while the harvesting of the product is initiated. A closed single step process using a 0.2 µm filter, HPH can generate the same product titer in half the time or twice the product compared to traditional Fed-Batch at no additional time.
Whether your intended application is mAb, rProteins, lentivirus, enzymes, or vaccines, HPH can boost productivity and deliver more product, faster.
Repligen solutions help overcome key challenges in High Productivity Harvest, with hands-on process and implementation consultation from global Field Applications Specialists.
High Productivity Harvest Workflow
Click on the workflow link to find productivity and throughput solutions you can implement today.
- Generate up to 2X increase in protein production without additional time, OR
- Transition to a single-step 0.2 µm filtered, closed system
Maintain a Fed-Batch process
HPH increases productivity and throughput while maintaining a fed batch process. No perfusion or media exchange of the N bioreactor is required, allowing the N bioreactor to maintain a single and discreet fed batch harvest point.
HPH yielded 5X more VCD
HPH achieved 20 million viable cell density (VCD) compared to 4 million for Fed-Batch. HPH viability was maintained above 100% over a period of 8 days. Fed-Batch viability began to drop after day 3 to approximately 75% at days 8-9. Higher VCD and viability translated to an increase in total product yield. HPH yielded approximately 1500 mg/day while the Fed-Batch process achieved 800-1000 mg/day in approximately 8-10 days.
Higher VCD and viability with HPH
Higher product titer with HPH
High Productivity Harvest Webinar
The webinar demonstrates how HPH works, presents initial results of up to a two-fold increase in protein production, and illustrates how you can apply HPH specific to your product need or facility design.