高产量收获
高产量收获 (HPH) 是 Repligen 开发的一种新的应用，可以完全无菌的方式收获补料分批生物反应器以便在捕获柱上处理，同时消除离心和深层过滤步骤。
High Productivity Harvest (HPH) uses an XCell ATF® Cell Retention Device after peak density is achieved in a typical Fed-Batch process. Fresh media is added in a set flow rate while the harvesting of the product is initiated. A closed single step process using a 0.2 µm filter, HPH can generate the same product titer in half the time or twice the product compared to traditional Fed-Batch at no additional time.
Whether your intended application is mAb, rProteins, lentivirus, enzymes, or vaccines, HPH can boost productivity and deliver more product, faster.
Repligen solutions help overcome key challenges in High Productivity Harvest, with hands-on process and implementation consultation from global Field Applications Specialists.
High Productivity Harvest Workflow
Click on the workflow link to find productivity and throughput solutions you can implement today.
提高产率
- Generate up to 2X increase in protein production without additional time, OR
- 可在更短的天数收获相同的滴度，提高产率
Improve processes
- 获得更健康的培养，同时降低工艺相关杂质
- 降低单元操作和原材料数量
- Transition to a single-step 0.2 µm filtered, closed system
- 收获料液可直接用于下游纯化
Maintain a Fed-Batch process
HPH increases productivity and throughput while maintaining a fed batch process. No perfusion or media exchange of the N bioreactor is required, allowing the N bioreactor to maintain a single and discreet fed batch harvest point.
HPH yielded 5X more VCD
HPH achieved 20 million viable cell density (VCD) compared to 4 million for Fed-Batch. HPH viability was maintained above 100% over a period of 8 days. Fed-Batch viability began to drop after day 3 to approximately 75% at days 8-9. Higher VCD and viability translated to an increase in total product yield. HPH yielded approximately 1500 mg/day while the Fed-Batch process achieved 800-1000 mg/day in approximately 8-10 days.
Higher VCD and viability with HPH
- 相比标准补料分批，使用HPH补料分批可使活细胞密度提高4倍
- 活性维持接近100%
Higher product titer with HPH
- 相比传统补料分批，产物提高2倍
- 在与补料分批相同（或更少）的天数内收获
High Productivity Harvest Webinar
The webinar demonstrates how HPH works, presents initial results of up to a two-fold increase in protein production, and illustrates how you can apply HPH specific to your product need or facility design.
工作原理
以下为HPH工艺操作的典型示例，其包括三个主要步骤：开始补料分批工艺，连接细胞截留装置开始高产量收获，之后开始抽液降低生物反应器内体积。HPH的启动时间是灵活的，且取决于工艺。收获可以在与传统补料分批相同的时间框架内完成，或者，生物反应器运行可以根据工艺和设施条件适当延长，以进一步提高产量。
步骤A：工艺开始
正常开始补料分批工艺。
步骤B：连接细胞截留设备（CRD），开始高产量收获
当达到峰细胞密度后，连接细胞截留装置(CRD)，并开始以较低的流速泵入新鲜的培养基。随着新鲜培养基的泵入，开始慢慢收获产物。
步骤C：抽液
运行的最后一天，停止添加新鲜培养基，开始降低生物反应器内的体积。
步骤D：工艺结束
HPH补料分批可以在与传统补料分批相同的时间内结束，或延长生产运行，以进一步提高产量。
注意：在本示例中，培养基置换在第10天开始，但也可根据工艺情况进行调节。
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